摘要
背景:研究发现,间充质干细胞来源的外泌体具有抑制炎症、促进皮肤细胞增殖和血管再生、减少肌成纤维细胞积累的作用,表明间充质干细胞主要通过旁分泌方式修复损伤,给皮肤损伤治疗提供了新方法。但应用犬脐带间充质干细胞来源外泌体治疗皮肤创伤的研究甚少。目的:研究犬脐带间充质干细胞来源的外泌体对犬皮肤创伤的修复作用,并探讨其作用机制。方法:选取年龄相近的健康中华田园犬12只,在背部正中肩胛骨下方皮肤做4 cm×4 cm正方形切口,然后随机分成外泌体组和模型组,每组6只。外泌体组在创口周围注射1 m L质量浓度为300 mg/L犬脐带间充质干细胞来源外泌体溶液,模型组注射等量生理盐水。造模前后每天观察犬的精神状况和创面愈合情况,定时检测血常规和创口面积,并于第1,2,4周进行病理组织观察。结果与结论:在造模后第1周,模型组出现了明显炎症反应,白细胞数量也较造模前升高了30%,而外泌体组炎症反应不明显,白细胞总数和中性粒细胞升高速度较模型组慢;两组的淋巴细胞数和红细胞数无明显差异。治疗组肉芽组织生长和伤口愈合速度较模型组快,但无显著性差异。结果提示,犬脐带间充质干细胞来源外泌体具有一定抑制炎症和促进皮肤创面愈合的作用。
BACKGROUND:Studies have found that mesenchymal stem cell-derived exosomes can inhibit inflammation,promote skin cell proliferation and angiogenesis,and reduce myofibroblast accumulation.In other words,the injury repair by mesenchymal stem cells is mainly implemented via paracrine mechanism,providing a new method for the treatment of skin injuries.However,there are few studies on the treatment of skin wounds using canine umbilical cord mesenchymal stem cell-derived exosomes.OBJECTIVE:To investigate the repair effect of umbilical cord mesenchymal stem cell-derived exosomes on canine skin wounds and the underlying mechanisms.METHODS:Twelve healthy dogs with the same age and growth status were selected.A 4 cm×4 cm square full-thickness incision was made under the medial scapula of the dog back and these 12 dog models were randomly divided into two groups,with 6 rats in each group.The treatment group was injected with 1 mL of saline containing 300 mg/L exosomes,while the model group was injected with the same volume of normal saline as a control.Before and after the modeling,the dog’s mental status and wound healing were observed daily.The blood routine and the wound area were measured regularly and the callus tissues were taken to make pathological observation in the 1st,2nd and 4th week after modeling.RESULTS AND CONCLUSION:In the 1st week,there was a marked inflammatory reaction in the model group,and the number of white blood cells was also increased by 30%compared with the baseline data.The inflammatory response in the treatment group was not obvious,and the number of white blood cells and neutrophils was increased slowly as compared with the model group.There was no significant difference in the number of lymphocytes and red blood cells between the two groups.The growth of granulation tissue and wound healing in the treatment group were faster compared with the model group,but no significant differences were detected.These results suggest that canine umbilical cord mesenchymal stem cell-derived exosomes,to so
作者
詹小舒
罗冬章
王丙云
卢瑜
冼伟杭
蓝奕琦
郭建波
罗惠娜
白银山
计慧琴
陈胜锋
陈志胜
刘璨颖
Zhan Xiao-shu;Luo Dong-zhang;Wang Bing-yun;Lu Yu;Xian Wei-hang;Lan Yi-qi;Guo Jian-bo;Luo Hui-na;Bai Yin-shan;Ji Hui-qin;Chen Sheng-feng;Chen Zhi-sheng;Liu Can-ying(School of Life Science and Engineering,Foshan University,Foshan 528231,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第25期4021-4027,共7页
Chinese Journal of Tissue Engineering Research
基金
广东省自然科学基金(2017A030313171)
广东省教育厅预防兽医学重点实验室项目(2014KTSPT037)
国家级大学生创新创业计划训练项目
2017年度佛山科学技术学院学术基金项目~~
