摘要
目的探讨血浆miRNA-21和miRNA-142-5p表达水平在肾细胞癌临床诊断中的价值。方法收集淮安市第二人民医院2015年7月~2017年10月的肾细胞癌患者和良性肾病患者血液标本,分别为55例和49例。应用实时荧光定量PCR法检测肾细胞癌患者和良性肾病患者血浆中miRNA-21和miRNA-142-5p的表达,并分析血浆miRNA-21和miRNA-142-5p表达量与肿瘤患者临床病理特征的相关性。构建受试者工作特征(ROC)曲线,评估这两个血浆miRNAs对于肾细胞癌的诊断效能。结果肾细胞癌患者血浆中miRNA-21和miRNA-142-5p的表达水平均明显高于良性肾疾病患者(P<0.01)。血浆miRNA-21的表达水平与肾细胞癌的临床分期显著相关(P<0.05),而血浆miRNA-142-5p的表达与肾细胞癌患者的临床病理特征无明显相关性(P>0.05)。55例肾细胞癌患者血浆中miRNA-21、miRNA-142-5p表达量无明显相关性(P>0.05)。血浆miRNA-21和miRNA-142-5p的ROC曲线下面积(AUC)分别为0.826(95%CI:0.794~0.912)和0.805(95%CI:0.744~0.919)。血浆miRNA-21诊断肾细胞癌的敏感性和特异性分别为81.8%和83.7%,血浆miRNA-142-5p诊断肾细胞癌的敏感性和特异性分别为80.0%和77.6%。而两种miRNAs联合检测,诊断肾细胞癌的敏感性提高至87.3%,但特异性略有下降,为75.5%。结论血浆中miRNA-21和miRNA-142-5p表达水平很有可能成为肾细胞癌诊断的潜在生物标志物。
Objective To investigate the clinical values of plasma miRNA-21 and miRNA-142-5p expression levels in the diagnosis of renal cell carcinoma(RCC).Methods Blood samples of patients with RCC and bebign renal disease from July 2015 to October 2017 in the Second People′s Hospital of Huai′an were collected,there were 55 cases and 49 cases respectively.The expression levels of plasma miRNA-21 and miRNA-142-5p were determined by fluorescence quantitative PCR in these patients.The correlations between the two miRNAs levels in plasma and clinicopathological characteristics of patients with RCC were analyzed.The diagnostic performance of each miRNA to distinguish RCC from benign renal disease was evaluated by the receiver operating characteristic(ROC)curve analysis.Results The levels of plasma miRNA-21 and miRNA-142-5p in RCC patients were higher than those in benign renal disease(P<0.01).There was no significant correlation between plasma miRNA-142-5p levels and clinicopathological characteristics of RCC patients(P>0.05),whereas the expression levels of plasma miRNA-21 was correlated with clinical stages(P<0.05).There was no significant correlation between the expression of miRNA-21 and miRNA-142-5p in the plasma of 55 patients with RCC(P>0.05).The area under ROC curve of the two miRNAs were 0.826(95%CI:0.794-0.912)and 0.805(95%CI:0.744-0.919).The sensitivity and specificity were 81.8%and 83.7%for miRNA-21,and 80.0%and 77.6%for miRNA-142-5p,respectively,for diagnosing RCC.And the sensitivity was increased to 87.3%with miRNA-21 in combination with miRNA-142-5p,despite slightly decreased specificity which was 75.5%.Conclusion These findings demonstrated that plasma miRNA-21 and miRNA-142-5p may serve as a promising biomarker in the diagnosis of RCC.
作者
李乾
姜福金
温儒民
王文天
LI Qian;JIANG Fujin;WEN Rumin;WANG Wentian(Clinical College,Xuzhou Medical University,Jiangsu Province,Xuzhou 221004,China;Depatrment of Urinary Surgery,the Second People′s Hospital of Huai′an,Jiangsu Procince,Huai′an 212001,China;The State Key Laboratory of Experimental Hematology,Institute of Hematology&Blood Disease Hospital,Chinese Academy of Medical Sciences,Tianjin 300020,China)
出处
《中国医药导报》
CAS
2018年第21期4-7,18,共5页
China Medical Herald
基金
国家自然科学基金青年科学基金项目(81500084)
