摘要
猪流行性腹泻的临床症状主要是腹泻、呕吐,哺乳仔猪患病后死亡率高,对猪群的健康构成了严重的威胁。该病诊断时与传染性胃肠炎较难分辨,实验室诊断技术的准确性和及时性,对于准确、快速地诊断该病发挥了非常重要的作用。目前用于该病的诊断方法主要有病原检测技术、血清中和试验、ELISA(酶联免疫吸附试验)法、荧光定量PCR技术、反转录酶链式反应(RT-PCR)检测法、多重PCR技术、胶体金免疫技术等。
General clinical signs of porcine epidemic diarrhea(PED)comprise of diarrhea,vomiting and high mortality in diseased suckling piglets.The prevalence of PED in pig farms poses a great threat to the porcine health.Clinically,differential diagnosis between PED and porcine transmissible gastroenteritis is always difficult.The accuracy and timeliness of laboratory diagnosis techniques exert a crucial role in accurate and rapid diagnosis of PED.Recently,the commonly used diagnosis methods of PDE are pathogen detection technique,serum neutralization test,ELISA assay,real-time fluorescence quantitative RT-PCR assay(RT-qPCR),reverse transcriptase-polymerase chain reaction technique(RT-PCR),multiplex PCR assay,immune colloidal gold technique and so on.
作者
王婧
刁小龙
牛蒙蒙
陈晓兰
张龙
WANG Jing;DIAO Xiao-long;NIU Meng-meng;CHEN Xiao-lan;ZHANG Long(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Zhongchong Sino Biotech Taizhou Co.,Ltd,Taizhou 225300,China)
出处
《畜牧与饲料科学》
2018年第7期105-108,共4页
Animal Husbandry and Feed Science
基金
江苏省农业三新工程项目(SXGC[2017]249)
泰州市科技支撑计划(农业)项目(TN201711)
江苏农牧科技职业学院院级项目(NSF201504-2
NSF201606)
