摘要
目的探讨核受体辅阻遏子(NCoR)与类固醇受体辅活化子-1(SRC-1)在子宫内膜重度不典型增生中的表达及意义。方法选择2014年1月至2017年2月收治的60例子宫内膜重度不典型增生患者作为观察组,选择同期行内膜活检的30例子宫内膜无病变对象作为对照组,均留取子宫内膜标本,采用免疫组化法观察子宫内膜组织NCoR、SRC-1、雌激素受体(ER)、孕激素受体(PR)表达情况,采用逆转录-聚合酶链反应(RT-PCR)法测定子宫内膜组织NCoR、SRC-1 m RNA表达。结果 (1)观察组SRC-1阳性率高于对照组(68.33%vs.6.67%)(P<0.05),其NCoR(20.00%vs.50.00%)、ER(13.33%vs.76.67%)、PR表达阳性率(18.33%vs.80.00%)低于对照组,对比差异有统计学意义(P<0.05);(2)观察组子宫内膜组织SRC-1 m RNA水平明显高于对照组,其NCoR m RNA水平低于对照组,对比差异有统计学意义(P<0.05);(3)子宫内膜重度不典型增生患者内膜组织SRC-1与ER表达呈负相关(r=-0.465,P<0.05),而NCoR与PR表达水平呈正相关(r=0.478,P<0.05)。结论子宫内膜重度不典型增生患者内膜组织NCoR表达水平较低,且与PR表达呈正相关,SCR-1表达水平较高,与ER负相关,推测NCoR、SRC-1两者均可通过介导雌激素调节,影响内膜组织增生,参与子宫内膜癌变过程。
Objective To investigate the expression and significance of nuclear receptor corepressors(NCoR)and steroid receptor coactivator-1(SRC-1)in severe endometrial atypical hyperplasia.Methods A to-tal of 60 patients with severe endometrial atypical hyperplasia treated in our hospital from January 2014 to February 2017 were selected as the observation group,and 30 subjects without endometrial lesions who underwent endometri-al biopsy in the same period were selected as control group.The endometrial specimens were taken from all sub-jects.The expression of NCoR,SRC-1,estrogen receptor(ER)and progesterone receptor(PR)in endometrial tis-sues was determined by immunohistochemistry,and the expression of NCoR and SRC-1mRNA in endometrial tis-sues was determined by the reverse transcription polymerase chain reaction(RT-PCR).Results(1)The positive rate of SRC-1 in the observation group was higher than that in the control group(68.33%vs.6.67%)(P<0.05),and the positive expression rates of NCoR(20.00%vs.50.00%),ER(13.33%vs.76.67%)and PR(18.33%vs.80.00%)were lower than those in the control group(P<0.05);(2)The levels of SRC-1 mRNA in the endometri-al tissues of the observation group were significantly higher than those in the control group,and the levels of NCoR mRNA were lower than those in the control group(P<0.05);(3)There was a negative correlation between the expression of SRC-1 and the expression of ER in the endometrial tissues of patients with severe atypical hyperplasia(r=-0.465,P<0.05),while the expression of NCoR was positively correlated with the expression of PR(r=0.478,P<0.05).Conclusions The expression level of NCoR is relatively lower in the endometrial tissues of patients with severe atypical hyperplasia,and it is positively correlated with the expression of PR.However,the expression level of SRC-1 is relatively higher,and it is negatively correlated with ER.It is speculated that both of NCoR and SRC-1 can affect endometrial hyperplasia and participate in the process of endometrial canceration through mediating es
作者
李翠芬
刘燕燕
杨宇峰
霍炽文
LI Cuifen;LIU Yanyan;YANG Yufeng;HUO Zhiwen(Department of Gynecology,the Third People′s Hospital of Dongguan,Dongguan 523326,China)
出处
《实用医学杂志》
CAS
北大核心
2018年第13期2204-2208,共5页
The Journal of Practical Medicine
基金
东莞市社会发展项目(编号:2015108101032)
