摘要
目的通过研究合成性激素的关键酶17β-HSD1在肾脏中的表达,探讨肾脏是否具备合成性激素的作用。方法基于无促卵泡生成素(FSH)、黄体生成素(LH)培养基和有FSH、LH培养基两种条件下,Western blotting、放射免疫分析法分别检测培养24、48 h后肾脏细胞中17β-HSD1的表达和性激素的分泌情况。结果培养24 h后,大鼠肾脏细胞能够表达少量的17β-HSD1蛋白(0.1843±0.076),同时能够分泌少量的雌二醇、孕酮和睾酮(分别为3.30±3.78 nmol/L,62.60±12.33 pmol/L和22.12±3.36 nmol/L),而在FSH和LH的共同刺激下,大鼠肾脏细胞17β-HSD1蛋白的表达量明显升高(1.6651±0.044,P<0.01),同时分泌雌二醇、孕酮和睾酮的量也显著增加(分别为8.50±2.64 nmol/L,117.80±9.79 pmol/L和45.04±4.39 nmol/L,均P<0.05),培养24h和48 h上述指标均无明显差异(P>0.05)。结论大鼠肾脏细胞中有17β-HSD1的表达,并且在FSH和LH的共刺激下能够稳定分泌性激素,提示肾脏组织具备合成性激素的能力,丰富了肾脏的内分泌功能。
Objective To investigate the expression of 17 beta-hydroxysteroid dehydrogenase type 1（17β-HSD1） in the kidney of rats and explore the capacity of the kidney for synthesizing sex hormones. Methods The expressions of 17- HSD1 and sex hormones were detected by Western blotting and radioimmunoassay in rat renal cells in primary cultured for 24 and 48 h in the presence or absence of follicle-stimulating hormone（FSH） and luteinizing hormone（LH）. Results After cell culture for 24 h, the primary rat renal cells expressed a low level of 17β- HSD1（0.1843 ± 0.076）, which increased to 1.6651 ± 0.044（P〈0.01） in response to co- stimulation by FSH and LH. Low levels of estradiol, progesterone and testosterone were also detected in rat renal cells（3.30±3.78, 62.60±12.33, and 22.12±3.36, respectively）, and co-stimulation of FSH and LH significantly increased their levels to 8.50 ± 2.64, 117.80 ± 9.79, and 45.04 ± 4.39, respectively（P〈0.05）. The levels of these hormones showed no significant differences between cells cultured for 24 h and 48 h（P〈0.05）. Conclusion The rat renal cells express 17β-HSD1 and are capable of stably secreting sex hormones in response to co-stimulation with FSH and LH, suggesting the capacity of the rat kidneys for synthesizing sex hormones. These findings enrich the understanding of the endocrine function of the kidney.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2016年第2期265-268,共4页
Journal of Southern Medical University
基金
陕西中医管理局基金(2007-84)
关键词
肾脏
17β-HSD1
内分泌
kidneys
17 beta-hydroxysteroid dehydrogenase type 1
sex hormones
endocrine
