摘要
本试验旨在研究21日龄断奶对仔猪肠道形态、肠道通透性及肠黏膜屏障的影响。采用2×3双因子完全随机试验设计,组别(哺乳组、断奶组)和日龄(22、24和28日龄)为2个主效应。选取6窝体况相近的健康大白仔猪,每窝选取6头平均体重为(6.1±0.2)kg的仔猪,随机分为2组,分别为断奶组和哺乳组,每组18头,分别于22、24和28日龄时屠宰,测定其生长性能、肠道形态、肠道通透性及肠黏膜屏障。结果表明:1)28日龄时,断奶组仔猪的末重和平均日增重均极显著低于哺乳组(P<0.01)。2)组别和日龄对仔猪的空肠隐窝深度和绒毛高度/隐窝深度、回肠绒毛高度有极显著交互作用(P<0.01);断奶组的空肠、回肠隐窝深度极显著高于哺乳组(P<0.01),空肠、回肠绒毛高度和绒毛高度/隐窝深度极显著低于哺乳组(P<0.01);哺乳组28日龄时的空肠绒毛高度/隐窝深度极显著高于22和24日龄时(P<0.01),24日龄时的回肠绒毛高度极显著高于22日龄时(P<0.01)。3)组别和日龄对仔猪的空肠黏膜二胺氧化酶(DAO)活性有显著交互作用(P<0.05),断奶组的空肠、回肠黏膜DAO活性显著低于哺乳组(P<0.05)。4)组别和日龄对仔猪空肠黏膜闭锁蛋白(occludin)的mRNA表达量有极显著交互作用(P<0.01);空肠黏膜闭锁小带蛋白1(ZO-1)和哺乳组空肠黏膜occludin的mRNA表达量随着日龄的增加先升高后降低(P<0.05);与哺乳组相比,断奶组回肠黏膜ZO-1、occludin和24日龄时空肠黏膜occludin的mRNA表达量显著降低(P<0.05);24日龄时回肠黏膜ZO-1的mRNA表达量显著高于22日龄时(P<0.05)。5)组别和日龄对仔猪空肠黏膜肿瘤坏死因子α(TNF-α)的mRNA表达量有显著交互作用(P<0.05);断奶组24和28日龄时空肠黏膜TNF-α的mRNA表达量显著高于哺乳组(P<0.05),空肠黏膜白细胞介素10(IL-10)的mRNA表达量显著低于哺乳组(P<0.05)。回肠黏膜白细胞介素1β(IL-1β)的mRNA表达量随着日龄的增加而降低(P<0.05)�
This experiment was conducted to investigate the effects of weaning at 21 days of age on intestinal morphology,permeability and mucosal barrier of piglets.According to 2×3 double?factor completely randomized test design,groups(nursing group and weaning group)and days of age(22,24 and 28 days of age)as the two main factors.Healthy Yorkshire piglets with similar body condition were selected from 6 litters,and 6 piglets with average body weight of(6.1±0.2)kg were selected from each litter and assigned to 2 groups(nursing group and weaning group)with 18 piglets per group.Piglets were slaughtered at 22,24 and 28 days of age,respectively,and growth performance,intestinal morphology,permeability and mucosal barrier were measured.The results showed as follows:1)the final body weight and average daily gain of piglets in weaning group were significantly lower than that in nursing group at 28 days of age(P<0.01).2)There were signifi?cant interactions of group and days of age on crypt depth and the ratio of villus height to crypt depth(V/C)of jejunum and villus height of ileum of piglets(P<0.01).Crypt depth of jejunum and ileum in weaning group was significantly higher than that in nursing group(P<0.01),and villus height and V/C of jejunum and ileum in weaning group were significantly lower than that in nursing group(P<0.01).V/C of jejunum in nursing group at 28 days of age was significantly higher than that at 22 and 24 days of age(P<0.01),and villus height of ileum at 24 days of age was significantly higher than that at 22 days of age(P<0.01).3)There was significant interaction of group and days of age on the activity of diamine oxidase(DAO)of jejunal mucosa of piglets(P<0.05),and the activity of DAO of jejunal and ileal mucosa in weaning group was significantly lower than that of nursing group(P<0.05).4)There was significant interaction of group and days of age on the mRNA expression of occludin of jejunal mucosa of piglets(P<0.01).The mRNA expression of occludin of jejunal mucosa in nursing group and zonuls occludens(Z
作者
夏冰
孟庆石
解竞静
吴维达
冯潇慧
唐湘方
张宏福
XIA Bing;MENG Qingshi;XIE Jingjing;WU Weida;FENG Xiaohui;TANG Xiangfang;ZHANG Hongfu(State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第6期2097-2108,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(31501960)
中国农业科学院创新工程(ASTIP-IAS07)
国家自然科学基金(31672428).
关键词
断奶仔猪
肠道形态
肠道通透性
肠黏膜屏障
weaning piglets
intestinal morphology
intestinal permeability
intestinal macosal barrier
