摘要
本文分析了桑树SSR的实验过程:基因组DNA提取、引物筛选、PCR反应体系优化、引物退火温度选择、凝胶配制、电泳和凝胶染色、显色,并指出了这些步骤中需要注意的问题,为SSR技术在桑树研究中的应用提供基础。
In order to provide a foundation for the application of SSR(Simple Sequence Repeat)technology in mulberry research,this paper analyzes the experimental process of SSR used in mulberry:genomic DNA extraction,primer screening,optimization of SSR PCR reaction system,primer annealing temperature selection,gel preparation,electrophoresis,gel staining and color development,and points out the issues that should be paid attention to in these steps.
作者
刘玲
傅晓
柯皓天
吕银
LIU Ling;FU Xiao;KE Hao-tian;LU Yin(Silk Engineering Technology Research Center of Sichuan Province,Chengdu 610031,China;Silk Science Research Institute of Sichuan Province,Chengdu 610031,China)
出处
《蚕学通讯》
2018年第1期17-20,共4页
Newsletter of Sericultural Science
基金
四川省科技计划项目(2017JY0277)
关键词
桑树
SSR
实验技术要点
mulberry
SSR
key point in experiment technology
