摘要
在完全混合流反应器中接种亚硝化颗粒污泥,通过分阶段使用连续流和序批式运行方式,成功启动了全自养生物脱氮(CANON)工艺,并对反应器性能、污泥形态与活性、微生物菌群结构的变化规律进行了深入分析。结果表明,基于初始连续流运行获得的良好基质比,序批式阶段的高氨氮负荷和高溶解氧条件可有效促进污泥浓度与活性的增长,使得反应器在最终连续流状态下的总氮去除负荷达到了1.75 kg·(m^3·d)^(-1)。运行期间,颗粒污泥的密实度和沉降性能均得到改善。由Miseq高通量测序的结果可知,CANON颗粒污泥具有相对较高的微生物多样性。对应于总氮比去除速率0.24 g·(gVSS·d)^(-1),Nitrosomonas(好氧氨氧化菌)与Candidatus Kuenenia(厌氧氨氧化菌)丰度比值约为3:1。少量贫营养型亚硝酸盐氧化菌对CANON工艺没有显著影响。
In order to start up a completely autotrophic nitrogen removal over nitrite(CANON)process,the continuous flow and sequencing batch modes were used in stages to operate the completely mixed flow reactor inoculated with nitrosation granular sludges.The variations of reactor performance,sludge morphology and activity,and the microbial community structure were investigated in details.Results showed that sequencing batch operation could promote the increases in both sludge concentration and reaction activity at high influent ammonium loading rate and high dissolved oxygen level,on basis of an appropriate nitrogen substrate ratio obtained in the initial continuous flow stage.Then a superior total nitrogen removal rate of 1.75 kg·(m3·d)-1 was achieved in the subsequent continuous flow mode.During the operation period,the compactness and settling property of granules were improved obviously.And Miseq high-throughput pyrosequencing analysis revealed that CANON granular sludge showed a relative higher microbial diversity.Corresponding to the specific nitrogen removal rate of 0.24 g·(g VSS·d)-1,the abundance ratio between aerobic and anaerobic ammonium oxidation bacteria was around 3 to 1,in terms of gene Nitrosomonas and Candidatus Kuenenia,respectively.In addition,few oligotrophic nitrite oxidation bacteria in the granules would not affect the efficiency of CANON process significantly.
作者
陈希
王建芳
钱飞跃
高军军
沈耀良
周丰
CHEN Xi;WANG Jianfang;QIAN Feiyue;GAO Junjun;SHEN Yaoliang;ZHOU Feng(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,Jiangsu,China;National and Local Joint Engineering Laboratory of Resource Utilization Technology of Municipal Sewage,Suzhou 215009,Jiangsu,China;Jiangsu High Education Collaborative Innovation Center of Water Treatment Technology and Material,Suzhou 215009,Jiangsu,China)
出处
《化工学报》
EI
CAS
CSCD
北大核心
2018年第4期1695-1702,共8页
CIESC Journal
基金
国家自然科学基金项目(51308367
51578353)
江苏高校优势学科建设工程资助项目
江苏省研究生科研创新计划项目(SJLX16-0560)
苏州科技大学研究生科研创新计划项目(SKCX16-032
SKCX16-022)~~
关键词
废水
CANON工艺
固定化
连续流
序批式
曝气
菌群结构
waste water
CANON process
immobilization
continuous flow mode
sequencing batch mode
aerobic
microbial community structure
