摘要
以室温培养的单级PN-ANAMMOX(PN/A)颗粒污泥为对象,基于颗粒污泥的全自养脱氮工艺,研究在低温条件下处理低浓度氨氮废水的脱氮效能及微生物群落结构.结果表明,在(15±1)℃条件下,维持氨氮负荷在1.29kg·(m^3·d)^-1,进水氨氮质量浓度从70 mg·L^-1逐级降低至40 mg·L^-1,溶解氧比剩余氨氮(DO/TAN)维持在0.22~0.25,总氮去除率可维持在(85±4)%,出水总氮平均质量浓度约为8.9 mg·L^-1,运行期内无亚硝酸氧化菌(nitrite-oxidizing bacteria,NOB)显著增殖,Nitrospira丰度小于1%.淘洗絮体污泥和控制低DO/TAN值可作为抑制NOB增殖的有效调控策略.全自养脱氮颗粒污泥在低温低基质条件下运行,颗粒粒径会变小,颜色由棕红色变为棕黄色.PS总量略有下降,PN/PS的比值稳定在2.5~3.浮霉菌门(Planctomycetes)和变形菌门(Proteobacteria)在微生物系统中占主导,污泥中存在CandidatusKuenenia和CandidatusBrocadia两种厌氧氨氧化菌属.
A single-stage PN-ANAMMOX(PN/A)granular sludge cultured at room temperature was used to investigate the completely autotrophic nitrogen removal efficiency and microbial community structure of low-strength wastewater based on the completely autotrophic nitrogen removal over nitrite in granular sludge at a low temperature.The results showed that at the low temperature(15±1)℃,the ammonia nitrogen load was maintained at 1.29 kg·(m^3·d)^-1,and the ammonia nitrogen concentration in the injection was gradually reduced from 70 mg·L^-1 to 40 mg·L^-1.DO/TAN was controlled at 0.22-0.25.The total nitrogen removal rate was maintained at(85±4)%,and the average TN concentration in the effluent was 8.9 mg·L^-1.There was no significant proliferation of nitrite-oxidizing bacteria(NOB)during the operation period,and the Nitrospira abundance was less than 1%.Elutriation of the floc sludge and the control of low DO/TAN values can be used as effective control strategies to inhibit NOB proliferation.Through completely autotrophic nitrogen removal over nitrite in granular sludge operated under low-temperature and low-substrate conditions,the particle size became smaller,and the color changed from brown red to brown yellow.The total amount of PS decreased slightly,and the ratio of PN/PS stabilized at 2.5-3.0.Planctomycetes and Proteobacteria dominated the community,and CandidatusKuenenia and CandidatusBrocadia were two AMX bacteria in the sludge.
作者
刘雨馨
王建芳
钱飞跃
陈倩苗
乔伟
沈聪
LlU Yu-xin;WANG Jian-fang;QIAN Fei-yue;CHEN Qian-miao;QIAO Wei;SHEN Cong(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;College of Tianping,SuzhouUniversity of Science and Techmology,Suzhou 215009,China;National and Local Joint EngineeringLaboratory of Municipal Sewage Resouree Uilization Technology,Suzhou 215009,China;Jiangsu High Education CollaborativeInnovation Center of Water Treatment Technology and Material,Suzhou 215009,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2020年第9期4161-4168,共8页
Environmental Science
基金
国家自然科学基金项目(51878430,51578353)
江苏省高等学校自然科学研究项目(18KJ B610019)
苏州市科技计划项目(SS 201834)
苏州科技大学研究生科研创新计划项目(SKCX 18_003)
江苏省高校优势学科建设工程项目
环境工程江苏省重点专业类项目。
关键词
颗粒污泥
全自养脱氮
低温
低基质
DO/TAN
NOB抑制
granular sludge
completely autotrophic nitrogen removal
low temperature
low-strength
DO/TAN
NOB repressure