摘要
为提高大肠杆菌基因工程菌E.coli-p ET21a高密度培养生产L-抗坏血酸-2-葡糖苷酶产量,采用摇瓶培养的方法,通过考察培养液菌体量和酶产量,筛选适用于E.coli-p ET21a液体深层发酵的培养基。50 L发酵罐采用适用于工业化大生产的搅拌、供氧、p H控制和过程补料方式,进行液体深层发酵放大培养,大幅度提高酶产量。通过优化发酵过程控制p H和诱导温度,发酵液酶活提高至88 U/m L,达到摇瓶培养的10.1倍。研究结果对大肠杆菌基因工程菌高密度培养,高效表达生物酶有重要的参考价值。
The high-density culture of recombinant bacteria E.coli-pET21a was investigated to improve the production of L-ascorbic acid-2-glucosidase.The optimal culture medium for E.coli-Pet21a was determined through evaluating the biomass and enzyme yield by shake-flask culture.The production of enzyme got huge increase by enlarging high cell density fermentation to 50 L tank,which was suitable for industrial production by adjusting the process of mixing,oxygen supplying,pH controlling and the supplement feeding.After optimized the fermentation pH and the induction temperature,the enzyme activity of the fermentation broth reached 88 U/mL,which was 10.1 times higher than that of shaking flask culture.The research results provided important references for the production of high-density fermentation of E.coli genetic engineering bacteria.
作者
余玉奎
桂馨
李平
李敏
LI Min(School of Life Science and Technology,Tongji University,Shanghai 200092,China)
出处
《工业微生物》
CAS
2018年第2期29-34,共6页
Industrial Microbiology
