摘要
解淀粉芽孢杆菌Bs-18是一株对黄瓜白粉病具有显著防效的生防菌株,为深入研究此菌株的生防作用机理,本研究应用电击法构建了携带转座子Tn YLB-1的穿梭质粒p Mar A转化Bs-18的突变体文库。通过对转座温度和转座时间的筛选,筛选出50℃高温诱导16 h可获得最大转座成功率,并对突变体进行了PCR验证。Bs-18突变体文库的构建为研究其生防功能基因并深入揭示其生防作用机制奠定了基础。
Bacillus amyloliquefaciens Bs-18 is a biocontrol strains against cucumber powdery mildew with significant control effect.In this paper,the mutant library of Bs-18 was constructed with the shuttle plasmid pMarA carrying transposon TnYLB-1 by the electric shock method to deeply study the molecular mechanism of the biocontrol strain.Through screening transpositional temperature and time,the maximum transpositional success rate was obtained after induced at 50℃for 16 hours.The mutants were verified by PCR.The construction of Bs-18 mutant library laid a foundation for further study on its biocontrol function genes and mechanism.
作者
孙淑琴
孙冰冰
杨秀荣
张惟
霍建飞
Sun Shuqin;Sun Bingbing;Yang Xiurong;Zhang Wei;Huo Jianfei(Tianjin Institute of Plant Protection,Tianjin 300381,China)
出处
《山东农业科学》
2018年第4期12-15,共4页
Shandong Agricultural Sciences
基金
天津市自然科学基金项目"解淀粉芽孢杆菌Bs-18生防相关基因的鉴定及功能分析"(16JCYBJC29500)
关键词
突变体文库
转座子
生防功能基因
Mutant library
Transposon
Biocontrol function genes
