摘要
目的对比研究IHC检测乳腺癌组织中HER2蛋白表达与FISH检测HER2基因扩增的一致性,探讨HER-2基因表达与患者临床病理特征的相关性。方法收集2010—2015年乳腺癌根治术后癌组织标本463例,应用FISH检测标本中HER2基因表达、IHC检测HER2、ER、PR、Ki67、P53的蛋白表达,分析HER2基因与蛋白表达的一致性和相关性及其与患者临床病理特征的关系。结果463例乳腺癌标本中HER2基因扩增率为35.64%(165/463),HER2蛋白表达阳性率为26.35%(122/463);HER2蛋白表达、、+和-的标本中HER2基因扩增率分别为91.50%、10.00%、8.27%、7.14%;HER2基因扩增及蛋白表达与病理学分级(r=0.140、r=0.310,均P<0.01)、肿块大小(r=0.103、P<0.05,r=0.499,P<0.01)、有无淋巴结转移(r=0.175、r=0.220,均P<0.01)及Ki67表达(r=0.220、r=0.331,均P<0.01)呈正相关,与ER(r=-0.406、r=-0.382,均P<0.01)、PR表达(r=-0.610、r=-0.635,均P<0.01)呈负相关。结论 FISH和IHC两种方法检测HER2表达具有较好的一致性及相关性,但HER2蛋白表达组与HER2基因扩增间的一致性较低,提示HER2蛋白表达者须进一步进行FISH检测;HER2表达与Ki67、ER、PR、淋巴结转移、肿块大小及病理学分级之间存在相关性。
Objective To investigate the expression of HER2 in breast carcinomas by performing immunohistochemistry(IHC)and fluorescent in situ hybridization(FISH),and to analyze the relationship between HER2 expression and clinicopathological characteristics.Methods The expression of HER2 gene was detected by FISH and the expression of HER2,ER,PR,Ki67 and P53 protein was measured by IHC in 463 breast cancer samples collected from 2010 to 2015.The consistency between HER2 gene and protein expression and the relationship between HER2 expression and clinicopathological characteristics were analyzed.Results HER2 gene amplification rate and protein expression rate were 35.64%(165/463)and 26.35%(122/463),respectively.Furthermore,HER2 gene amplification rates were 91.50%,10.00%,8.27%and 7.14%in IHC HER2,,+and-samples,respectively.The gene amplification and protein expression of HER2 were positively correlated with pathological grade(r=0.140,r=0.310;P<0.01),tumor size(r=0.103,P<0.05;r=0.499,P<0.01),lymph node metastasis(r=0.175,r=0.220;P<0.01)and Ki67 expression(r=0.220,r=0.331;P<0.01),but negatively correlated with ER(r=-0.406,r=-0.382;P<0.01)and PR expression(r=-0.610,r=-0.635;P=0.01).Conclusion The IHC and FISH have a good consistency and correlation for detecting HER2 expression.However,there is a low consistency between HER2 protein expression and gene amplification in HER2 cases,suggesting that the HER2 should be further detected by FISH.The expression of HER2 is associated with Ki67,ER,PR,lymph node metastasis,tumor size and pathological grade.
作者
杨雅洁
孙艳花
YANG Ya-jie;SUN Yan-hua(Department of Pathology,Shenzhen Second People’s Hospital,Shenzhen 518035,China)
出处
《南昌大学学报（医学版）》
CAS
2018年第1期55-59,64,共6页
Journal of Nanchang University：Medical Sciences
基金
深圳市科技计划项目(JCYJ20150330102720174)
关键词
乳腺癌
HER2
免疫组织化学
荧光原位杂交
breast cancer
HER2
immunohistochemistry
fluorescent in situ hybridization
