摘要
目的研究2型糖尿病患者压疮局部ATP酶活性及凋亡相关蛋白表达。方法选取2型糖尿病合并压疮患者43例,其中Ⅲ期压疮21例,Ⅳ期压疮22例,排除近1个月内使用可能影响ATP酶药物的患者(洋地黄类、钙离子拮抗剂等),患者换药时取伤口周围皮肤组织标本,另择20例健康志愿者作为对照组,检测ATP酶活性及Bcl-2、Baxx信使RNA及蛋白表达。结果与对照组相比,Ⅲ期压疮组ATP酶活性降低,Bcl-2表达减少,Bax表达增多;与Ⅲ期压疮组相比,Ⅳ期压疮组ATP酶活性降低更为显著,Bcl-2表达显著减少,Bax表达显著增多。结论 2型糖尿病患者压疮可能与ATP酶活性降低及凋亡相关蛋白表达增强相关。
Objective To explore the ATPase activity and apoptotic-associated protein expression of chronic pressure ulcers patients with type 2 diabetes.Methods A total of 43 type 2 diabetes patients with pressure sores were chosen,among whom there were 21 cases withⅢpressure sores and 22 cases withⅣpressure sores,the patients who may use ATPase in the last one month(digitalis,calcium antagonists,etc.).Another Other 20 healthy volunteers were chosen as control group.ATPase activity and apoptotic associated protein expression were detected.Results Compared with the control group,the ATPase activity in the stageⅢpressure sore group was decreased,Bcl-2 expression was decreased,and the expression of Bax was increased;compared with the stageⅢpressure ulcer group,the ATPase activity in the stage IV pressure sore group was more significantly reduced,Bcl-2 was significantly decreased and Bax expression was significant increased.Conclusion Type 2 diabetic chronic pressure ulcers might be relevant to the decreased ATPase activity and up regulated apostosis.
作者
李峥璟
柏素萍
黄佳敏
孙辉
周筱琴
LI Zhengjing;BO Suping;HUANG Jiamin;SUN Hui;ZHOU Xiaoqin(Department of Burn and Plastic Surgery,Affiliated Hospital of Jiangsu University, Zhenjiang,Jiangsu,212001)
出处
《实用临床医药杂志》
CAS
2018年第8期21-23,27,共4页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金(81471903)
关键词
2型糖尿病
压疮
ATP酶活性
凋亡相关蛋白
type 2 diabetes
chronic pressure ulcers
ATPase activity
apoptosis related proteins
