摘要
目的通过检测大肠癌组织及大肠正常黏膜组织中miR-10b和HOXD10m RNA的表达情况,并分析其与患者临床病理特征的关系。方法采用real-time PCR技术检测53例大肠癌组织及其5cm以远正常大肠黏膜组织中miR-10b和HOXD10m RNA的表达水平,采用免疫组化SP法检测53例大肠癌组织及5c m以远正常大肠黏膜组织中HOXD10蛋白的表达。结果在53例大肠癌组织中,34例(65.38%)mi R-10b的表达升高,miR-10b相对表达量在大肠癌组织中为1.672(1.167~1.945),大肠正常黏膜组织中为0.648(0.215~0.946)(P<0.05);在53例大肠癌组织中,32例(60.38%)HOXD10m RNA表达下降,HOXD10m RNA相对表达量在大肠癌中为0.341(0.134~0.963),大肠正常黏膜组织中为0.810(0.524~1.027)(P<0.05)。在53例大肠癌组织中,HOXD10蛋白表达阳性20例(37.7%),而在53例大肠正常黏膜组织中,HOXD10蛋白表达阳性41例(77.4%)(P<0.05)。大肠癌患者mi R-10b的表达在有无淋巴结转移情况和不同临床分期之间者有统计学差异(均P<0.05),而在不同年龄、性别、肿瘤大小、浸润深度者之间均无统计学差异(均P>0.05),HOXD10m RNA的表达在有无淋巴结转移和不同临床分期者之间有统计学差异(均P<0.05),而在不同年龄、性别、肿瘤大小、浸润深度者之间差异均无统计学意义(均P>0.05)。在大肠癌组织中,miR-10b与HOXD10m RNA的表达呈负相关(P<0.05)。结论大肠癌患者中miR-10b和HOXD10m RNA的异常表达与大肠癌的侵袭和转移相关,miR-10b可成为大肠癌患者生物治疗的新靶点。
Objective To investigate the expression of miR-10b and HOXD10mRNA in colorectal cancer tissues and to analyze their relation to clinical pathological characteristics.Methods Fifty three samples of colorectal cancer and pericancerous mucosal tissue were collected.The real-time PCR was used to detect the expression of miR-10b and HOXD10mRNA,and immunohistochemical SP method was used to detect the expression of HOXD10 protein in cancer and normal tissues.SPSS21.0 statistical software was applied for data analysis.Results In 53 colorectal cancer patients,miR-10b expression was elevated in 34 cases(65.38%);the relative expression of miR-10b was 1.672(1.167-1.945)and 0.648(0.215-0.946)in colorectal cancer and normal mucosa tissues,respectively(P<0.05).The expression of HOXD10mRNA was reduced in 32 cases(60.38%);the relative expression of HOXD10mRNA was 0.341(0.134-0.963)and 0.810(0.524-1.027)in colorectal cancer and normal mucosa tissues,respectively(P<0.05).The expression of HOXD10 protein was detected in 20 samples of cancer tissue(37.7%)and 41 samples of normal mucosa(77.4%)(P<0.05).The expression of miR-10b and HOXD10mRNA was significantly associated with lymph node metastasis and clinical stages(P<0.05),but not associated with age,sex,tumor sizes,local invasion.There was a negative correlation between miR-10b and HOXD10mRNA expression(P<0.05).Conclusion In colorectal cancer,the abnormal expression of miR-10b and HOXD10mRNA is related to invasion and metastasis of the cancer.The study indicates that miR-10b might be used as a novel target for biotherapy in colorectal cancer patients.
作者
余铭
施正超
薛迪新
陈澄亮
陈积贤
贺新伟
金凯
孙利敏
YU Ming;SHI Zhengchao;XUE Dixin(Department of Surgical Oncology,the Third Affiliated Hospital of Wenzhou Medical University,Rui'an 325200,China)
出处
《浙江医学》
CAS
2018年第6期568-572,共5页
Zhejiang Medical Journal
基金
温州市科技计划项目(Y20140384)
