摘要
目的分析中国新型布尼亚病毒分离株的基因型分布和进化规律,了解分子流行病学特征。方法利用MEGA6.0软件,对GenBank数据库收录的中国地区分离株的完整S片段基因序列进行分析,构建系统进化树,并分析毒株间的核苷酸和氨基酸的同源性。结果在GenBank数据库收集到248株中国地区新型布尼亚病毒分离株,主要为人源毒株232株,占93.55%,核酸和氨基酸序列同源性分别为94.9%~96.8%和91.4%~95.2%;系统进化树分析显示中国地区流行优势株为C2基因亚型,占58.06%;5种亚型的核苷酸序列和氨基酸序列同源性为94.9%~96.8%、91.4%~95.2%,同亚型毒株核苷酸序列和氨基酸序列同源性为96.3%~99.1%、96.8%~98.6%。与汉坦病毒属和白蛉病毒属亲缘较远,毒株核苷酸序列同源性分别为39.3%~40.2%和47.3%~48.1%,氨基酸序列同源性13.6%~14.4%和22.5%~23.9%。人源毒株与宿主动物分离株高度同源,核苷酸序列和氨基酸序列同源性分别为96.3%~97.1%和94.0%~95.7%。结论我国近年来新型布尼亚病毒流行毒株以基因亚型C2为主,存在多种基因亚型间循环交替流行。
Objective To analyze the genotype distribution and genetic evolution of Severe fever with thrombocytopenia syndrome virus(SFTSV)isolated in China;to understand molecular epidemiological characteristic of SFTSV.Methods MEGA 6.0 software was used to analyze the segment S gene sequences of isolated virus strains in China after retrieving complete sequences from the GenBank nucleotide database.The phylogenetic tree was constructed and the homology of nucleotide and amino acids were calculated.Results A total of 248 virus isolated strains were aquired from GenBank originated from China,mainly from human samples,accounted for 93.55%;the homology of nucleotide acids and amino acids were from 94.9%to 96.8%and 91.4%to 95.2%;phylogenetic analysis showed that C2 subtype was the dominant strain in China,accounted for 58.06%.The nucleotide sequences and amino acid sequences were 94.9%to 96.8%and 91.4%to 95.2%homologous among 5 subtypes,respectively.The homology of nucleotide sequences and amino acid sequences among same subtype strains ranged from 96.3%to 99.1%and 96.8%to 98.6%,respectively.Compared with hantavirus and phlebovirus strains,the homology of nucleotide sequences and amino acid sequences of isolated SFTSV strains were 39.3%-40.2%,47.3%-48.1%and 13.6%-14.4%and 22.5%-23.9%,respectively;isolates from human were highly homdogus to host animals,which had 96.3%-97.1%and 94.0%-95.7%homology for nucleotide sequences and amino acids sequences,respectively.Conclusion Subtype C2 was the dominant SFTSV strain,multi-subtypes co-circulated in China for last years.
作者
刘秀兰
姚学君
管书慧
姜仁杰
LIU Xiu-lan;YAO Xue-jun;GUAN Shu-hui;JIANG Ren-jie(ancheng Municipal Center for Disease Prevention And Control,Jiangsu Yancheng 224100,China)
出处
《江苏预防医学》
CAS
2018年第2期119-121,共3页
Jiangsu Journal of Preventive Medicine
关键词
新型布尼亚病毒
基因特征
序列分析
流行病学特征
Severe fever with thrombocytopenia syndrome virus
Genetic characteristics
Sequence analysis
Epidemiological characteristics
