摘要
采用沉淀聚合法,将不同配比的温敏性单体N-异丙基丙烯酰胺(NIPAm)、电负性单体丙烯酸(Aac)、疏水性单体N-叔丁基丙烯酰胺(t BAM)和交联剂N,N'-亚甲基双丙烯酰胺(Bis)共聚,得到一系列聚合物水凝胶纳米颗粒(NPs),改变表面活性剂的用量,得到不同粒径的NPs。通过扫描电镜和动态光散射对NPs形貌和粒径进行表征,并对NPs吸附溶菌酶进行动力学研究。实验结果表明,所得到的NPs呈圆球状、粒径均匀且分布窄。当优化单体比例为Aac 20%,t BAM 40%,NIPAm 38%,Bis 2%时,NPs吸附性能最佳;NPs粒径从386.20 nm减小至77.25 nm,其吸附性能逐渐增大。在优化的条件下制备的水凝胶纳米颗粒,对溶菌酶的吸附在5 min内可以达到平衡,吸附率可以达到68.7%,利用NPs温敏性可实现对溶菌酶的脱附,具有良好重复利用性。
Hydrogel nanoparticles(NPs)were synthesized by precipitation polymerization method with N-isopropyl acrylamide(NIPAm),acrylic acid(Aac),N-tert butyl acrylamide(tBAM)and N,N'-methylene bisacrylamide(Bis)as thermosensitive monomer,negative monomer,hydrophobic monomer and crosslinker,respectively.The morphology of the resulting NPs was characterized by scanning electron microscopy(SEM),and the size and the particle size distribution were investigated by dynamic light scattering(DLS).The dynamics test was also carried out to investigate adsorption property of NPs.The results showed that NPs was spherical with uniform particle size and narrow distribution.NPs had the best adsorption performance to lysozyme when the monomer molar ratio was optimized to Aac 20%,tBAM 40%,NIPAm 38%and Bis 2%.Meanwhile,when the particle size of NPs decreased from 386.20 nm to 77.25 nm,the adsorption capacity increased gradually.The adsorption rate could reach up to 67.8%within 5 minutes.The thermosensitive of NPs provided a new candidate for the adsorption and separation of lysozyme with good reusability.
作者
王哲
薛敏
孟子晖
吉田田
谢腾升
WANG Zhe;XUE Min;MENG Zi-Hui;JI Tian-Tian;XIE Teng-Sheng(School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 102488,China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第3期317-323,共7页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(No.21375009)资助~~
