摘要
目的观察当归补血汤主要吸收成分对2型糖尿病模型GK大鼠肾保护作用及可能机制。方法 UPLC-MS/MS定性定量吸收入血活性成分(ABCs),确定ABCs组成,定量1g母方中ABCs浓度。GK大鼠按血糖随机分为模型组、当归补血汤组及吸收成分(ABCs)组,每组10只,给药组分别灌胃当归补血汤(4g/kg)、吸收入血的生物活性成分(剂量相当于母方含量,即咖啡酸5.36mg/kg,芒柄花素2.68mg/kg,毛蕊异黄酮2.56mg/kg,阿魏酸1.36mg/kg,黄芪甲苷0.45mg/kg,丁苯酞0.16mg/kg,蒿本内酯0.16mg/kg),10只普通Wistar大鼠作为空白对照,给予等量生理盐水,每日1次,连续给药28d。观察指标:(1)肾脏系数、尿总蛋白和尿白蛋白;(2)肾组织氧化应激;(3)病理切片观察肾脏组织形态。结果当归补血汤及ABCs均能够改善糖尿病大鼠的肾脏肥大,显著降低糖尿病模型大鼠尿总蛋白、尿白蛋白和肾脏MDA水平(P<0.05),显著升高肾脏总SOD活力(P<0.05),且对肾脏组织病理形态学有保护作用;与当归补血汤组比较,ABCs作用不如汤剂显著。结论 ABCs能够代表母方当归补血汤发挥对GK大鼠的肾保护作用,机制涉及氧化应激。母方中除ABCs外,其微量元素、氨基酸、多糖等其他成分也同时发挥着药效作用。
OBJECTIVE To observe renal protective effect and possible mechanism of absorbed bioactive compounds(ABCs)of Danggui Buxue Decoction on GK rats.METHODS The qualitative and quantitative analysis of ABCs of Danggui Buxue Decoction were performed by UPLC MS/MS and the composition of ABCs were determined.Then the ABCs concentration of 1 g mother decoction was quantified.GK rats were randomly divided into model group,decoction group and ABCs group(n=10).Treatment groups were administered Danggui Buxue Decoction(4 g/kg)or ABCs(equal to the content of decoction,caffeic acid 5.36 mg/kg,formononetin 2.68 mg/kg,calycosin 2.56 mg/kg,ferulic acid 1.36 mg/kg,astragaloside 0.45 mg/kg,butyphthalide 0.16 mg/kg,ligustilide 0.16 mg/kg),once a day for 28 d.10 Wistar rats were chosen for control group and treated with vehicle(saline).Observation index:urinary total protein and urinary albumin,oxidative stress in kidney and the pathological observation of kidney.RESULTS Danggui Buxue Decoction and ABCs significantly decreased urinary total protein,urinary albumin of GK rats and the level of MDA of kidney(P<0.05),significantly increased the activity of SOD of kidney(P<0.05)and displayed a protective effect on kidney pathomorphology.CONCLUSION ABCs can represent Danggui Buxue Decoction play a renal protective effect on GK rats,the mechanism involving oxidative stress.However,except for ABCs,the trace elements,amino acids,polysaccharides and other ingredients in mother decoction also play significant roles.
作者
薛梅
卞勇
周俊杰
刘雅琳
黄熙
XUE Mei;BIAN Yong;ZHOU Jun-jie;LIU Ya-lin;HUANG Xi(Basic Medical College,Nanjing University of Chinese Medicine,Nanjing,210023,China;Institute of Common Disease and Depression,Nanjing University of Chinese Medicine,Nanjing,210023,China;Experimental Animal Center of Nanjing University of Chinese Medicine,Nanjing,210023,China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2018年第2期190-193,共4页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏高校优势学科(中西医结合)建设工程资助项目
