摘要
背景:急性移植物抗宿主病是调节T细胞介导的炎症性疾病,通过改善机体CD4^+CD5^+Treg及其相关炎症因子水平对预防及治疗急性移植物抗宿主病具有一定的意义。目的:探讨粒细胞集落刺激因子对恶性血液疾病患儿异基因造血干细胞移植后CD4^+CD5^+Treg及相关细胞因子水平的影响。方法:选取70例接受异基因造血干细胞移植的血液疾病患儿,其中45例供者移植前经粒细胞集落刺激因子动员为粒细胞集落刺激因子组,另25例直接采集骨髓为非粒细胞集落刺激因子组,分别于异基因造血干细胞移植后4周采集外周血,另选取60例健康体检儿童为对照组,应用流式细胞仪及ELISA法测定各组外周血调节T细胞水平及血清白细胞介素35、干扰素γ水平。根据异基因造血干细胞移植后是否发生急性移植物抗宿主病将70例血液疾病患儿分为急性移植物抗宿主病阳性组及急性移植物抗宿主病阴性组,分析调节T细胞及细胞因子水平变化与急性移植物抗宿主病的关系。结果与结论:(1)血液疾病患儿CD4^+CD5^+Treg占CD4^+T细胞比例、CD4^+CD5^+Treg占CD4^+FoxP 3^+T细胞比例及血清白细胞介素35水平低于对照组(P<0.05),而干扰素γ水平均高于对照组(P<0.05);(2)粒细胞集落刺激因子组CD4^+CD5^+Treg占CD4^+T细胞比例、CD4^+CD5^+Treg占CD4^+FoxP 3^+T细胞比例及血清白细胞介素35水平低于非粒细胞集落刺激因子组(P<0.05),而干扰素γ水平高于非粒细胞集落刺激因子组(P<0.05);(3)粒细胞集落刺激因子组移植后4周急性移植物抗宿主病阳性7例(16%),非粒细胞集落刺激因子组移植后4周急性移植物抗宿主病阳性10例(40%),两组患者急性移植物抗宿主病发生率差异有显著性意义(P=0.022);(4)急性移植物抗宿主病阴性组CD4^+CD5^+Treg占CD4^+T细胞比例、CD4^+CD5^+Treg占CD4^+FoxP 3^+T细胞比例及血清白细胞介素35水平高于急性移植物抗宿主病阳性组(P<0.05),而�
BACKGROUND:Acute graft-versus-host disease(aGVHD)is an inflammatory disease mediated by regulatory T cells.It is of certain significance to prevent and treat aGVHD by improving the levels of CD4+CD5+regulatory T cells(CD4+CD5+Treg)and related inflammatory factors in the body.OBJECTIVE:To investigate the effect of granulocyte colony stimulating factor(G-CSF)on CD4+CD5+regulatory T cells(CD4+CD5+Treg)and cytokines in children with hematological malignancies after allogeneic hematopoietic stem cell transplantation(allo-HSCT).METHODS:Seventy children with hematological malignancies undergoing allo-HSCT were enrolled.G-CSF was mobilized in the 45 cases(G-CSF group)before allo-HSCT transplantation.The other 25 cases were assigned to non-G-CSF group.Peripheral blood samples were collected at 4 weeks after allo-HSCT.Another 60 healthy children were selected as control group.The levels of T cells,serum interleukin-35(IL-35)and interferon-γ(INF-γ)were measured by flow cytometry and ELISA,respectively.According to whether aGVHD occurred after allo-HSCT,the 70 cases were divided into aGVHD positive group and aGVHD negative group.The relationship between the changes of T cells and cytokines with aGVHD was analyzed.RESULTS AND CONCLUSION:(1)The percentage of CD4+CD5+Treg in CD4+T and CD4+FoxP3+T cells as well as serum IL-35 level in children with hematological diseases were lower than those in the control group(P<0.05),while the levels of INF-γwere significantly higher than that in the control group(P<0.05).(2)The percentage of CD4+CD5+Treg in CD4+T cells and CD4+FoxP3+T cells as well as serum IL-35 level in the G-CSF group were lower than those in the non-G-CSF group(P<0.05),while the levels of INF-γwas higher than that in the non-G-CSF group(P<0.05).(3)There were 7 cases(16%)of aGVHD in the G-CSF group and 10 cases(40%)of aGVHD in the non-G-CSF group at 4 weeks after allo-HSCT,and the incidence of aGVHD was significantly different between the G-CSF and non-G-CSF groups(P=0.022).(4)The percentage of CD4+CD5+Treg in CD4+T
作者
向金峰
Xiang Jin-feng(Pediatric Department of Hematology,Nanyang Central Hospital,Nanyang 473000,Henan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第1期89-94,共6页
Chinese Journal of Tissue Engineering Research
基金
河南省南阳市科技成果(kj650322)~~
关键词
造血干细胞移植
粒细胞集落刺激因子
血液病
细胞因子类
组织工程
Hematopoietic Stem Cell Transplantation
Granulocyte Colony-Stimulating Factor
Hematologic Diseases
Cytokines
Tissue Engineering
