摘要
目的了解中国不同地区市售鸡肉源大肠杆菌耐药性和耐药特征,为抗生素的合理使用提供参考。方法对全国8个省市的超市或农贸市场随机采集的1 152份鸡肉样品进行大肠杆菌的分离鉴定,采用琼脂稀释法对15种抗生素进行药敏试验。结果鸡肉大肠杆菌的分离率为65.97%;大肠杆菌对15种抗生素具有不同程度的耐药,萘啶酮酸、阿莫西林-克拉维酸、四环素、甲氧苄啶-新诺明和氨苄西林的耐药率高达60%以上;而对加替沙星、头孢哌酮和阿米卡星的抗性均低于20%。大肠杆菌多重耐药为70.53%,其中以8重耐药最多10.26%。结论大肠杆菌存在区域性耐药差异,并且多重耐药现象严重,存在通过食物链传播给人的风险。
The aim of this study was to determine the prevalence and antibiotic susceptibility of Escherichia coli from retail raw chickens in different provinces of China.A total of 1 152 whole chicken samples were collected and screened for the prevalence of E.coli,and then the E.coli isolates were further tested for the antimicrobial susceptibility using agar dilution method.Results showed that the overall positive rate for E.coli in retail chickens was 65.97%(760/1 152);resistance was most common to nalidixic acid(66.84%),followed by amoxicillin-clavulanic acid(66.05%),tetracycline(65.00%),trimethoprim-sulfamethoxazole(63.16%),ampicillin(60.66%),amoxicillin(51.32%),streptomycin(50.39%),chloramphenicol(48.32%),kanamycin(38.29%),gentamicin(26.31%),ciprofloxacin(25.79%)and cefoxitin(21.05%).The last were gatifloxacin,cefoperazone and amikacin(each<20%).Overall,70.53%of the isolates were resistant to at least three antimicrobials.A large proportion of multidrug resistant isolates were resistant to 8 kinds of antimicrobials(10.26%).No strain was resistant to 15 kinds of antimicrobials.Furthermore,isolates recovered from different regions exhibited different resistance levels to most antimicrobials.Our findings indicate that retail chicken in China was commonly contaminated with E.coli,and many E.coli strains exhibited multiple drug resistance.Presence of multiple drug E.coli in raw chickens may pose a potential threat to human health.
作者
李穗霞
席美丽
王盼盼
吴倩
吴聪明
沈建忠
付骋宇
张鹏飞
王新
LI Sui-xia;XI Mei-li;WANG Pan-pan;WU Qian;WU Cong-ming;SHEN Jian-zhong;FU Cheng-yu;ZHANG Peng-fei;WANG Xin(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Shaanxi Entry-Exit Inspection and Quarantine Bureau,Xi'an 710068,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2018年第2期158-164,共7页
Chinese Journal of Zoonoses
基金
国家自然基金-新疆联合基金项目(No.U1703119)
国家自然科学基金(No.31271858)
中国博士后科学基金(No.2015M582711)
陕西省社会发展科技攻关项目(No.2016SF-251)联合资助~~
关键词
市售鸡肉
大肠杆菌
耐药性
多重耐药性
retail raw chicken
Escherichia coli
antibiotic resistance
multidrug resistance
