摘要
提高赤霉病抗性已成为我国小麦主产区的重要育种目标之一。Fhb1是抗性最强且最稳定的抗赤霉病基因,阐明其在我国小麦育种中的应用及传递路径,对抗赤霉病育种有重要意义。本研究通过分析229份小麦品种(系)Fhb1区段内PFT(pore-forming toxin-like)、HC(HCBT-like defense response protein)和His(histidine-rich calcium-binding protein)基因的多样性与赤霉病抗性的关系,发现PFT-I/His-I为抗病单倍型。基因检测和系谱分析表明,中国小麦品种所含Fhb1至少有2个来源,分别为苏麦3号和宁麦9号,并以后者为主。本研究开发的诊断性标记PFT-CAPS和His-InDel可有效用于Fhb1的分子标记辅助育种。
Enhancing resistance to Fusarium head blight(FHB)has become one of important breeding objectives in the major wheat-growing regions in China.A prominent locus Fhb1 conferring stable FHB resistance with the largest effect is the major source of resistance in wheat breeding.Understanding the distribution and putative donor of Fhb1 in Chinese wheat cultivars will facilitate the application of this gene and thus benefit FHB resistance breeding in China.Haplotype analysis of PFT(pore-forming toxin-like),HC(HCBT-like defense response protein)and His(histidine-rich calcium-binding protein)genes in the Fhb1 region of 229 wheat cultivars and advanced lines indicated that PFT-I/His-I was a resistant haplotype.Both pedigree and marker(or sequence)information revealed that Fhb1 in Chinese wheat cultivars was mainly derived from Sumai 3 and Ningmai 9,in which Ningmai 9 was the major donor.The Fhb1 diagnostic markers PFT-CAPS and His-InDel developed in this study can be used effectively in marker-assisted selection for improving FHB resistance.
作者
朱展望
徐登安
程顺和
高春保
夏先春
郝元峰
何中虎
ZHU Zhan-Wang;XU Deng-An;CHENG Shun-He;GAO Chun-Bao;XIA Xian-Chun;HAO Yuan-Feng;HE Zhong-Hu(Institute of Crop Science,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,China;Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China;Institute of Agricultural Sciences of Lixiahe District in Jiangsu Province,Yangzhou 225007,Jiangsu,China;CIMMYT-China Office,c/o CAAS,Beijing 100081,China)
出处
《作物学报》
CAS
CSCD
北大核心
2018年第4期473-482,共10页
Acta Agronomica Sinica
基金
本研究由国家重点研发计划项目(2016YFD0101802,2016YFE0108600),国家自然科学基金项目(31301306)和湖北省技术创新专项(2016AHB022)资助。
关键词
小麦
赤霉病
Fhb1
诊断性标记
分子标记辅助选择
bread wheat
Fusarium head blight
Fhb1
diagnostic marker
marker-assisted selection
