摘要
目的观察程序性死亡受体1(PD-L1)在乳腺癌组织中的表达情况,并探讨其与乳腺癌患者细胞免疫的相关性。方法收集迁安市人民医院乳腺癌石蜡标本114例,免疫组织化学技术检测PD-L1在乳腺肿瘤组织中的表达情况,分析PD-L1的表达与临床病理特征的关系;流式细胞仪检测PD-L1阴性、PD-L1阳性两组患者外周血淋巴细胞亚群中CD3^+、CD3^+CD4^+、CD3^+CD8^+、CD3^-CD19^+、CD3^-CD16^+CD56`+细胞的水平及CD4^+/CD8^+比值。结果在114例乳腺癌标本中,有35例患者PD-L1阳性表达,表达率为30.7%,PD-L1表达与肿瘤大小、组织学分级、Ki-67高表达相关(P<0.05);PD-L1阴性表达患者细胞免疫状态优于PD-L1阳性表达者(P<0.05)。结论 PD-L1的表达与乳腺癌更差的临床病理特征密切相关,其机制可能与PDL1阳性表达患者的细胞免疫被破坏相关。
Objective To observe the expression of PD-L1 in breast cancer tissue and to investigate its correlation with cellular immunity.Methods One hundred and fourteen paraffin-embedded specimens of breast cancer were collected from the Qian′an Municipal People′s Hospital.The expression of PD-L1 in breast tumor tissue was detected by immunohitochemical technique,then the relationship between PD-L1 expression and tumor clinicopathological characteristics was analyzed;the levels of peripheral blood CD3+,CD3+CD4+,CD3+CD8+,CD3-CD19+,CD3-CD16+CD56+cells and CD4+/CD8+in the patients with PD-L1 negative and PD-L1 positive of the two groups were detected by the flow cytometry.Results Among 114 cases of breast cancer specimen,35 cases(30.7%)were PD-L1 expression positive with the expression rate of 30.7%.The PD-L1 expression was correlated with the tumor size,histological grade and Ki-67 high expression(P<0.05);the cellular immunity status in the patients with PD-L1 negative expression was better than that in the patients with PD-L1 positive expression(P<0.05).Conclusion The PD-L1 expression is closely correlated with the poorer clinicopathological characteristics of breast cancer,its mechanism may be correlated with the disruption of cellular immunity in the patients with PD-L1 positive expression.
作者
王立东
徐卫国
杨鑫
李蕊洁
谌娜娜
郭红辉
赵跃鹏
WANG Lidong;XU Weiguo;YANG Xin;LI Ruijie;CHEN Nana;GUO Honghui;ZHAO Yuepeng(Department of Oncological Surgery,Affiliated Hospital,North China University of Science and Technology,Tangshan,Hebei 063009,China;Department of Tumor Chemoradiotherapy,Qian′an Municipal People′s Hospital,Qian′an,Hebei 064400,China;Department of Pathology,Qian′an Municipal People′s Hospital, Qian′an,Hebei 064400,China;Department of Surgery,Chengde County Hospital,Chengde,Hebei 067400,China)
出处
《重庆医学》
CAS
2018年第9期1207-1209,共3页
Chongqing medicine
