摘要
目的探讨CD34、基质金属蛋白酶(MMP-9)、血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)在骨巨细胞瘤(GCTB)组织中的表达及其临床意义,为GCTB的诊断与治疗提供临床依据。方法将2010年1月至2017年1月收治的80例GCTB手术患者纳入研究,采用免疫组化染色法检测GCTB组织中CD34、MMP-9、VEGF、PCNA的表达水平,分析其与GCTB患者年龄、性别、肿瘤大小、Jaffe分级以及预后之间的相关性。结果CD34、MMP-9、VEGF、PCNA的阳性表达与GCTB患者年龄、性别无明显相关性(P>0.05),与肿瘤大小、Jaffe分级、预后有显著相关性(P<0.05)。CD34与MMP-9、VEGF、PCNA的表达呈正相关(r=0.311,0.424,0.401;P=0.015,0.025,0.025)。结论 CD34、MMP-9、VEGF、PCNA在GCTB的发生发展中有重要作用,检测其表达水平对GCTB的诊断和预后具有一定的临床意义。
Objective To explore the expression and clinical significance of CD34,matrix metalloproteinase(MMP)-9,vascular endothelial growth factor(VEGF),proliferating cell nuclear antigen(PCNA)in giant cell tumor of bone(GCTB),so as to provide clinical basis for the diagnosis and treatment of GCTB.Methods A total of 80 patients with GCTB treated from January 2010 to January 2017 were enrolled in this study.The expression of CD34,MMP-9,VEGF and PCNA in these patients was assayed by immunohistochemistry staining,and relations of expression of CD34,MMP-9,VEGF,PCNA with age,gender,tumor size,Jaffe grading and prognosis were analyzed.Results Positive expression of CD34,MMP-9,VEGF and PCNA showed no significant correlation with age or gender for GCTB patients,while levels of CD34,MMP-9,VEGF and PCNA were related to tumor size,Jaffe grading and prognosis;CD34 levels showed positive correlation to the expression of MMP-9,VEGF and PCNA(r=0.311,0.424,0.401;P=0.015,0.025,0.025).Conclusion CD34,MMP-9,VEGF,PCNA play important roles in the development and progression of GCTB,examination of expressions of these cytokines have certain clinical significances for the diagnosis and prognosis of GCTB.
作者
彭仲华
梁亮科
PENG Zhonghua;LIANG Liangke(Department of Orthopaedics Traumatology,Sanshui Hospital Affiliated to Foshan TCM Hospital,Foshan,Guangdong 528100,China)
出处
《中国骨科临床与基础研究杂志》
2017年第6期349-354,共6页
Chinese Orthopaedic Journal of Clinical and Basic Research
关键词
巨细胞瘤
骨
细胞因子类
抗原
CD34
基质金属蛋白酶9
血管内皮生长因子类
增殖细胞核抗原
肿瘤复发
局部
预后
Giant cell tumor of bone
Cytokines
Antigens,CD34
Matrix metalloproteinase 9
Vascular endothelial growth factors
Proliferating cell nuclear antigen
Neoplasm recurrence,local
Prognosis
