摘要
为研究复合酶预处理法对银杏叶总萜内酯浸出率的影响。本文采用单因素实验对银杏叶酶解预处理过程中的酶种类、酶用量、酶解时间、酶解温度、p H值五个关键因素进行探索,酶解后用20%乙醇-水溶液对银杏叶酶解液进行回流提取,LX-5型大孔吸附树脂对提取液进行纯化,浓缩干燥得银杏叶提取物产品。高效液相色谱法检测其总萜内酯含量。结果表明,银杏叶酶解预处理法的最佳工艺为:复合酶(纤维素酶∶果胶酶=1∶1),用量为银杏叶的1/300、酶解时间4.0 h、酶解温度50℃、初始p H值4.0。在该工艺条件下获得的银杏叶总萜内酯提取率为0.55%,银杏叶提取物中总萜内酯含量为8.96%,高于银杏叶提取物国际商务标准(总内酯含量≥6%)。说明复合酶预处理条件下银杏叶总萜内酯提取率高,研究为工业化生产高质量银杏叶提取物提供了理论依据。
To study the effects of compound enzyme pretreatment on the extraction rate of total terpene lactones in Ginkgo biloba leaves.Five key factors of enzyme type,enzyme dosage,enzymolysis time,enzymolysis temperature and pH value during the pretreatment of G.biloba were studied by single factor experiment.After enzymolysis,G.biloba extract was extracted with 20%ethanol solution,purified by LX-5 macroporous adsorption resin,concentrated and dried.Then,G.biloba extract product was obtained.High performance liquid chromatography was used to detect the contents of total terpene lactones.The results showed that the enzymolysis effect was the best when the ratio of cellulose to pectinase was 1∶1,the ratio of enzyme to G.biloba was 1∶300,the enzymolysis time was 4.0 h,the enzymolysis temperature was 50℃and the initial pH was 4.0.The extraction rate of total terpene lactone was 0.55%and the total terpene lactone content in G.biloba extract was 8.96%which was higher than G.biloba extract international business standards(total lactone content≥6%).The results showed that the extraction rate of total terpene lactones in G.biloba leaves was high under the condition of compound enzyme pretreatment.This study provided a theoretical basis for the industrial production of high quality G.biloba extract.
作者
张晓娟
赵正栋
张辰露
杨光
杜林峰
冯自立
ZHANG Xiao-juan;ZHAO Zheng-dong;ZHANG Chen-lu;YANG-Guang;DU Lin-feng;FENG Zi-li(School of Biological Science and Engineering,Shannxi University of Technology,Hanzhong 723001,China;Shannxi Yuchen Bio-Technology company,Chenggu 723003,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第3期429-433,共5页
Natural Product Research and Development
基金
陕西省科技厅项目(2014K14-03-03)
关键词
银杏叶
纤维素酶
果胶酶
银杏总萜内酯
提取率
Ginkgo biloba
cellulase
pectinase
total terpene lactones
extraction rate
