摘要
目的介绍树鼩清醒状态下尾静脉注射的单人操作方法与经验,提高尾静脉注射给药成功率。方法采用帆布手套套取和文件夹夹紧手套的方法固定树鼩,左手固定树鼩尾巴,右手持1 m L一次性注射器注射药物。结果本方法操作简单、经济实用、固定效果好、动物依从性好、给药成功率高。结论本方法与其他的尾静脉注射方法比较,有明显的优势。可单人操作,简便快速,省时省力;实验成本低,使用效果好,物美价廉;显著提高了树鼩尾静脉注射给药的成功率。
Objective To introduce a single person operation procedure for tail vein injection in conscious tree shrews,and to improve the success rate of injection.Methods The tree shrew was fixed by a canvas glove and a clamp.The tail of the tree shrew was fixed by the operator’s left hand,and the drug was injected by the right hand with a 1 mL disposable syringe.Results This method had the advantages of simple operation,economy and practicality,good fixation effect,high matching degree of animals and high success rate of drug administration.Conclusions Compared with other methods,this method has obvious advantages such as single person operation,convenient,time-saving and labor-saving.The cost is low and the effect is good,thus significantly improving the success rate of injection.
作者
何永旺
李娟
黄俭
周一卿
赵祥月
杨根梦
李桢
李娜
曾晓锋
HE Yongwang;LI Juan;HUANG Jian;ZHOU Yiqing;ZHAO Xiangyue;YANG Genmeng;LI Zhen;LI Na;ZENG Xiaofeng(School of Forensic Medicine,Kunming Medical University,Kunming 650500,China;Yunnan Key Laboratory of Major Infectious Diseases Vaccine Development;Yunnan Laboratory Tree Shrew Standardization and Applied Research Innovation Team,Kunming 650118)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第3期91-94,共4页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金地区基金项目(编号:81560303,81660310)
云南省科技人才和平台计划项目(编号:2017HC019)
云南省科技厅-昆明医科大学应用基础研究联合专项(编号:2016FE46)
云南省重大传染病疫苗研发重点实验室开放研究项目基金(编号:KF2015-01)
昆明医科大学毒品滥用·法医病理学科技创新团队(编号:CXTD201604)。
关键词
树鼩
尾静脉注射
单人操作
实验技能
实验动物
tree shrews
injection through tail vein
single person operation
experimental skills
laboratory animal sciences
