摘要
目的:研究水飞蓟油的体外抗氧化活性及其对D-半乳糖氧化损伤小鼠的保护作用。方法:通过测定水飞蓟油对二苯基苦味酰基苯肼自由基、羟自由基的清除能力及其总抗氧化力,研究其体外抗氧化活性;通过测定水飞蓟油对氧化损伤小鼠血清抗氧化酶活力以及其对小鼠肝组织形态的影响,研究其对氧化损伤小鼠的保护作用。结果:水飞蓟油具有一定的体外抗氧化能力,并与其质量浓度呈一定的正相关关系;水飞蓟油可显著提高小鼠血清中超氧化物歧化酶和谷胱甘肽过氧化物酶的活力及总抗氧化能力,可以保护小鼠肝细胞形态的完整性。结论:水飞蓟油具有一定的体外抗氧化活性,对D-半乳糖氧化损伤小鼠具有明显的保护作用。
Objective:To investigate the antioxidant activity in vitro and the protective effect of Silybum marianum seed oil on oxidative damage induced by D-galactose in mice.Methods:The scavenging capacity for DPPH and hydroxyl radicals and the total antioxidant capacity were determined to explore in vitro antioxidant activity of the seed oil.Furthermore,the effects of the seed oil on serum antioxidant enzyme activities and liver morphology were examined to evaluate its protective effect on oxidative damage in mice.Results:Silybum marianum seed oil had antioxidant capacity in vitro in a dose-dependent manner.It could significantly increase the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC)in mice serum and protect the integrity of liver cell morphology in mice.Conclusion:Silybum marianum seed oil has antioxidant activity in vitro and possesses a protective effect on D-galactoseinducedoxidative damage in mice.
作者
朱淑云
周越
肖香
董英
朱胜虎
ZHU Shuyun;ZHOU Yue;XIAO Xiang;DONG Ying;ZHU Shenghu(School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;Jiangsu Hengshun Group Co. Ltd., Zhenjiang 212143, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第5期234-238,共5页
Food Science
基金
江苏省高校自然科学研究面上项目(16KJB550001)
江苏省博士后科研资助计划项目(1601058A)
关键词
水飞蓟油
体外抗氧化
氧化损伤
抗氧化酶
Silybum marianum seed oil
in vitro antioxidant activity
oxidative damage
antioxidant enzyme
