摘要
【目的】本试验旨在研究赖氨酸对热应激后大鼠空肠上皮损伤的缓解作用及其自噬调控机制。【方法】利用人工气候箱以40℃下处理2h高温环境建立大鼠热应激模型,SD大鼠共18只,体重为(200±20)g,适应性饲养3d后随机分为对照组(C),热应激组(HS)和赖氨酸预处理后热应激各试验组(L-Lys+HS)。检测大鼠热应激前后体重变化,HE染色观察肠道上皮细胞的形态学损伤,RT-PCR检测MiRNA-23b以及自噬标志分子LC3B的表达变化。【结果】大鼠热应激预处理组中,赖氨酸饲喂高浓度(1.0%)对热应激后大鼠体重改善效果最佳;赖氨酸预饲喂大鼠后再热应激,大鼠空肠上皮损伤程度减轻;与自噬相关的LC3B的表达显著抑制,同时MiRNA-23b表达显著上升。【结论】预处理组中,1.0%高浓度赖氨酸的饲喂能够显著缓解热应激对大鼠空肠上皮的损伤,其中氨基酸通过MiRNA-23b对热应激引起的空肠上皮细胞的自噬有一定的缓解作用。
【Objective】This study was to investigate the remission effect of lysine on rat jejunal mucosal injury and autophagy.【Methods】The model of heat stress rat was established by constant temperature air table.The change of rat body weight was measured by analytical balance.The morphology of intestinal epithelial cell injury was observed by inverted microscope through HE staining.The expression levels of miRNA-23b and LC3B were detected by RT-PCR.【Result】Heat stress at40℃for2h is the best condition for rats.Feeding with high concentration lysine(1.0%)had the best effect on body weight improvement after heat stress.Through lysine pretreatment and heat stress on rats,the jejunum epithelial injury was relieved significantly.The expression of LC3B was significantly inhibited,while the expression of miRNA-23b was significantly increased.【Conclusion】Feeding with high concentration lysine could relieve rat jejunal injury caused by heat stress.The relief was through miRNA-23b inhibiting autophagy of jejunum epithelial cell caused by heat stress.
作者
刘艺林
黄丽卿
刘蓓桦
金娜
邹闻书
崔伟东
高倩
陈长增
刘凤华
LIU Yilin;HUANG Liqing;LIU Beihua;JIN Na;ZOU Wenshu;CUI Weidong;GAO Qian;CHEN Changzeng;LIU Fenghua(Animal Science and Technology College,Beijing University of Agriculture,Beijing102206,China;College of Veterinary Medicine,China Agricultural University,Beijing100094,China)
出处
《北京农学院学报》
2018年第1期54-58,共5页
Journal of Beijing University of Agriculture
基金
公益性行业(农业)科研专项经费项目(201403051-07)
