摘要
为了研究人参皂苷Re与精氨酸美拉德反应产物的抗氧化活性,采用人参皂苷Re与精氨酸在100℃反应4 h制备得到美拉德反应产物。HPLC分析产物中人参皂苷Re的结构变化,考察了反应体系的褐变程度,并测试了体系的总多酚含量、Cu^(2+)螯合能力、DPPH自由基清除能力,以及对Cu^(2+)诱导的HepG2细胞的抗氧化作用。结果显示,美拉德反应产物中人参皂苷Re部分转化为Rg2、Rg6和F4,美拉德反应产物的褐变程度、总多酚含量显著增加,与对照相比,美拉德反应产物对Cu^(2+)螯合能力和DPPH自由基清除能力显著提高,100~300μg/m L美拉德反应产物对Cu^(2+)诱导的HepG2细胞氧化应激具有显著的抑制能力。
The aim of the present study was to evaluate the important role of ginsenoside Re arginine Maillard reaction products(MRPs)in the free radical scavenging and against Cu2+induced oxidative stress in HepG2cells.Ginsenoside Re with arginine was heated at100℃for4h to prepare the MRPs.The MRPs was analyzed by HPLC,the browning level was assayed at420nm,total phenol content was assayed by the Folin Ciocalteu method and the result was illustrated as milligrams of GAE per gram of each sample tested.The change of Cu2+chelating activities and the scavenging effect on DPPH radical were also evaluated.Meanwhile,the antioxidant ability was also assayed in HepG2cells by using the Cu2+induced oxidative stress model.The results indicate that the ginsenoside Re is transformed into less polar ginsenosides such as Rg2,Rg6and F4during Maillard reaction,and the glucose molecule at carbon20is separated,the browning level and the total phenol increase obviously,the Cu2+chelating activity and DPPH radical scavenging activity are enhanced significantly.Comparing to the control,there is a3.16fold increase of oxidative stress of HepG2cells under the influence of Cu2+.The oxidative stress induced by Cu2+is only marginally inhibited by the treatment of ginsenoside Re or Re arginine mixture(not significant,p>0.05)but significantly reduced by the treatment with Re arginine MRPs in the concentration range of100to300μg/mL.Because of their high cell membrane permeability,the ginsenoside Re arginine MRPs increase the free radical scavenging acivity in HepG2cells,showing cellular antioxidant activity against Cu2+induced oxidative stress higher than that of Re.
作者
张儒
张变玲
易婷
贺婷
刘心怡
ZHANG Ru;ZHANG Bian ling;YI Ting;HE Ting;LIU Xin yi(College of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第11期1818-1823,共6页
Natural Product Research and Development
基金
湖南省自然科学基金(2016JJ2037
2017JJ3048)
湖南省教育厅基金(16C0394)
中国博士后特别资助和面上资助项目(2017T100601
2016M590746)
国家级大学生创新创业训练计划(201411342004)
