摘要
背景:文献报道将低强度脉冲超声联合关节腔内注射玻璃酸钠运用于家兔关节软骨缺损修复中效果理想,能促进缺损部位愈合,但是该结论尚未得到进一步证实。目的:进一步验证低强度脉冲超声联合关节腔内注射玻璃钠在家兔关节软骨缺损中的修复效果。方法:60只家兔采用4 mL/kg浓度为20%乌拉坦溶液注射麻醉,在股骨踝部作直径为3 mm、深为3 mm的关节软骨缺损模型,根据处理方法分为模型对照组、玻璃酸钠组和观察组。模型对照组建模成功后不采取任何措施处理,玻璃酸钠组关节腔内注射玻璃酸钠修复,观察组在玻璃酸钠组基础上联合低强度脉冲超声修复。大体观察关节兔软骨缺损部位的形态;比较3组家兔修复后苏木精-伊红染色、番红O染色及Wakitani组织学评分。结果与结论:①大体观察:模型对照组修复5周后缺损直径缩小,表面不平整且低于周围组织;玻璃酸钠组修复5后缺损部位与正常软骨组织边界清晰;观察组修复5周后缺损部位完全修复;②观察组家兔修复后2,5周Wakitani组织学评分,低于玻璃酸钠组和模型对照组(P<0.05);③观察组修复5周缺损部位细胞排列整齐;玻璃酸钠组修复5周后可见少许软骨区大量软骨样细胞;模型对照组修复后2,5周可见毛细心血管分布,排列不均匀;④观察组修复5周后缺损部位形态与正常软骨相似;玻璃酸钠组修复5周后深层蛋白多糖及软骨陷窝存在明显着色;模型对照组修复2,5周后深层染色偏白;⑤结果提示:低强度脉冲超声联合关节腔内注射玻璃钠在家兔关节软骨缺损中能取得理想修复效果。
BACKGROUND:As reported,the use of low-intensity pulsed ultrasound combined with intra-articular injection of sodium hyaluronate in rabbit articular cartilage defect repair is ideal,which can promote the healing of defect site.However,the conclusion has not been further confirmed.OBJECTIVE:To investigate the effect of low-intensity pulsed ultrasound combined with intra-articular injection of sodium hyaluronate in repairing articular cartilage defects in rabbits.METHODS:Sixty rabbits were anesthetized using20%urethane solution injection at a dose of4mL/kg,and a model of articular cartilage defect(3mm in diameter and3mm in depth)was made on the femoral ankle of each rabbit.Then,there were three groups in this study:model group with no intervention,sodium hyaluronate group with intra-articular injection of sodium hyaluronate,and observation group with intra-articular injection of sodium hyaluronate combined with low-intensity pulsed ultrasound.Gross observation,hematoxylin-eosin staining,safranin O staining and Wakitani scores were compared among groups after repair.RESULTS AND CONCLUSION:(1)At5weeks after repair,in the model group,the defect size was reduced with uneven surface that was lower than the surrounding tissues;in the sodium hyaluronate group,there was a clear boundary between the defect and surrounding cartilage tissues;in the observation group,the defect was completely repaired.(2)After2and5weeks,the Wakitani scores in the observation group were significantly lower than those in the other two groups(P<0.05).(3)After5weeks,in the observation group,the cells around the defect site arranged regularly,and in the sodium hyaluronate group,there were a large amount of cartilage-like cells in few cartilage regions.In the model group,there were irregularly arranged capillary blood vessels after2and5weeks.(4)After5weeks of repair,cartilage tissues with normal structure were found in the defect site in the observation group,and deeply stained proteoglycan and cartilage lacunae were observed in the sodium hyaluronat
作者
郝德峰
张鲁青
刘玉栋
Hao De-feng;Zhang Lu-qing;Liu Yu-dong(Department of Ultrasound, Weifang Yidu Central Hospital, Weifang 262500,Shandong Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2017年第30期4775-4780,共6页
Chinese Journal of Tissue Engineering Research
基金
山东省卫生厅基金资助项目(2015HZ026)~~
