摘要
以微波预糊化籼米淀粉为原料,自制RS_3型马铃薯抗性淀粉为晶种,研究RS_3型籼米抗性淀粉的晶种诱导-双酶复合法制备工艺。利用扫描电子显微镜对淀粉颗粒形貌进行表征并研究淀粉的抗酶解性。在单因素试验的基础上,固定其他酶解条件,以RS_3型籼米抗性淀粉产率为响应值,确定晶种添加量、异淀粉酶添加量、普鲁兰酶添加量和普鲁兰酶酶解时间作为影响产率的主要因素,进行Box-Behnken响应面优化试验。得到RS3型籼米抗性淀粉的最佳制备工艺条件为:晶种添加量5%、异淀粉酶添加量8 U/g、普鲁兰酶添加量8 U/g、普鲁兰酶酶解时间3.50 h。在此最佳制备工艺条件下,RS_3型籼米抗性淀粉产率为27.42%,RS3失去原有的淀粉颗粒形貌,表面变得粗糙,结晶结构致密,具有较强抗酶解能力。
In this research,RS3type resistant potato starch was prepared and used as seed crystal to investigate the preparation of RS3type resistant indica rice starch by the combined use of seed crystal and sequential hydrolysis with isoamylase and pullulanase.Scanning electron microscopy(SEM)was used to characterize starch granule morphology.Besides,we also studied the resistance of starches to enzymatic hydrolysis.Using the one-factor-at-a-time method,the amounts of seed crystal,isoamylase and pullulanase added and pullulanase hydrolysis time were identified as factors with a significant influence on the yield of RS3type resistant starch.These variables were optimized using response surface methodology with a Box-Behnken design.The maximum yield of RS3type resistant starch of27.42%was obtained under the following conditions:5%seed crystal,8U/g isoamylase,8U/g pullulanase and3.50h hydrolysis with pullulanase.The surface of the resistant starch granules was rough,which lost the original starch granule morphology,and the crystal structure of the resistant starch was compact.In addition,its resistance to enzymatic hydrolysis was strong.
作者
欧阳梦云
王燕
吴卫国
廖卢艳
OUYANG Mengyun;WANG Yan;WU Weiguo;LIAO Luyan(College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第18期244-250,共7页
Food Science
基金
湖南省重金属污染耕地治理修复机理及技术模式优化集成项目(农办财函[2015]38号)
关键词
晶种诱导
RS3型抗性淀粉
异淀粉酶
普鲁兰酶
seed crystal induction
RS3 type resistant indica rice starch
isoamylase
pullulanase
