摘要
以我国玉米育种中常用的16份自交系为材料,通过对SNAC(Stress-responsive NAM,ATAF1/2,CUC2)基因编码区及上游启动子区800 bp核苷酸序列进行测序,检测SNAC基因在不同杂种优势类群材料中的遗传变异。在12个SNAC基因中,其中有4个基因在上游800 bp区检测到遗传变异,有4个基因变异位点超过30个,多态性较高。虽然大多数SNAC基因变异以SNP(Single nucleotide polymorphism)为主,但在ZmNAC031467基因中检测到较多的插入缺失变异(In Del),达到基因总遗传变异的63.3%。通过PLACE软件对上游启动子有变异的4个基因进行3种耐逆结合元件的预测,结果显示4个基因均含有3种耐逆结合元件,但是基因突变对启动子结合元件的影响较小。再对检测到的遗传变异进行核苷酸多态性分析和中性检验,有7个SNAC基因核苷酸多态性较高,其中Zm NAC080308基因的多态性达到0.00962,推测这些基因在遗传漂移过程中受自然选择影响较大。利用t检验初步发现Zm NAC070395和Zm NAC080398基因的2个变异位点与耐旱相关性状关联,为进一步分析SNAC基因核苷酸变异与耐旱性状的关系提供一定的借鉴。
The coding regions and their upstream800bp promoter regions of SNAC genes(Sress-responsive NAM,ATAF1/2,CUC2)were sequenced in16maize inbred lines commonly used in China.Among12SNAC genes,genetic variations in promoterregion were only identified in four SNAC genes,and more than30variations were identified in four SNAC genes,showing higherpolymorphism in the four genes than other in SNAC genes.Although most of the SNAC genes were mainly SNP(Single nucleotidepolymorphism)mutations,more insertion/deletion mutations were detected in ZmNAC031467gene,reaching63.3%of thetotal genetic variations.The PLACE software was used to predict three kinds of stress-tolerant binding elements in SNAC gene,but little effect was found to be related with the variations.Additionally,high nucleotide polymorphisms were identified in sevenSNAC genes,especially with the highestπvalue of0.00962in ZmNAC030308,which suggested that they were greatly influencedby natural selection in the genetic drift.With the t-test,two mutations of ZmNAC070395and ZmNAC080398genes wereassociated with drought-tolerant traits,which provides references for further analysing the relationship between nucleotide variation in SNAC and drought tolerance traits.
作者
李国君
马艺文
徐丹阳
吴永波
宋洁
王楠
郝转芳
赵娟
LI Guo-Jun;MA Yi-Wen;XU Dan-Yang;WU Yong-Bo;SONG Jie;WANG Nan;HAO Zhuan-Fang;ZHAO Juan(College of Agronomy, Shanxi Agricultural University, Taigu 030801, China;Tonghua Academy of Agricultural Sciences, Tonghua 135007, China;Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
出处
《作物学报》
CAS
CSCD
北大核心
2017年第8期1128-1138,共11页
Acta Agronomica Sinica
基金
国家自然科学基金重大国际合作项目(31661143010)和面上项目(31271735)资助~~
关键词
玉米
SNAC基因
遗传变异
耐旱性
Zea mays L.
SNAC genes
Genetic variations
Drought tolerance
