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连翘叶中连翘酯苷A、芦丁和连翘苷提取纯化工艺优化 被引量:19

Simultaneous Extraction Purification of Forsythoside A, Rutin and Phillyrin from Forsythia suspensa Leaves
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摘要 对连翘叶提取液中同时分离连翘酯苷A、芦丁和连翘苷的提取纯化工艺进行优化。以连翘酯苷A、连翘苷和芦丁的质量浓度为指标,采用高效液相色谱法分析检测,比较不同型号的大孔吸附树脂和不同工艺条件对这3种活性成分的分离纯化能力。最佳工艺条件为:以AB-8大孔吸附树脂为吸附剂,药材-树脂比1.5∶1(g/g),上样流速2 BV/h,先用8 BV的水洗脱,再用6 BV的30%乙醇溶液和6 BV的50%乙醇溶液分别洗脱,洗脱流速3 BV/h;进一步采用C_(18)-硅胶柱反相层析法纯化连翘酯苷A,静置沉淀的方法纯化连翘苷和芦丁,纯化后连翘酯苷A和芦丁的纯度可达到97%以上,连翘苷纯度可达到95%以上。此工艺条件能得到高纯度的3种有效成分,为连翘叶资源的充分综合利用和产业化开发提供了参考。 In this study,we optimized the simultaneous separation and purification of forsythiaside A,phillyrin and rutinfrom an ethanolic extract of Forsythia suspensa leaves.These target compounds were quantitatively determined by highperformance liquid chromatography(HPLC).Different types of macroporous adsorption resin were compared for their abilitiesto separate and purify three active components from Forsythia suspensa leaves.AB-8resin was selected as the best one,and the optimum chromatographic conditions on this adsorbent were obtained as follows:sample-to-resin ratio,1.5:1(g/g);sample loading flow rate,2bed volumes(BV)/h;sequential elution with8BV of deionized water,6BV of30%ethanol and6BV of50%ethanol at a flow rate of3BV/h.Further purification of forsythiaside A was carried out using a C18-silica gelcolumn by reversed-phase chromatography,while purified phillyrin and rutin were obtained by precipitation after standing.Afterpurification,the purity of forsythiaside A and rutin was more than97%,and that of phillyrin was over95%.In conclusion,underthe optimized conditions,three effective components of high purity were obtained from Forsythia suspensa leaves.This study maybe helpful for the comprehensive utilization and industrialization exploitation of Forsythia suspensa leaves.
作者 孙建瑞 王大红 邱智军 原江锋 SUN Jianrui;WANG Dahong;QIU Zhijun;YUAN Jiangfeng(Luoyang Engineering and Technology Research Center of Microbial Fermentation, College of Food and Bioengineering,Henan University of Science and Technology, Luoyang 471023, China)
出处 《食品科学》 EI CAS CSCD 北大核心 2017年第14期200-205,共6页 Food Science
基金 国家自然科学基金青年科学基金项目(31401672) 国家自然科学基金联合基金项目(U1404307) 河南省科技厅重点攻关项目(162102110056) 洛阳市科技攻关项目(1401076A)
关键词 连翘叶 连翘酯苷A 芦丁 连翘苷 提取工艺 Forsythia suspensa leaves forsythoside A rutin phillyrin extraction conditions
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