摘要
茶氨酸合成酶(Theanine synthetase,TS)基因是茶树茶氨酸代谢过程中的关键酶基因。本研究以氨基酸含量差异明显的亲本及其杂交所得F_1子代为研究材料,克隆TS基因的c DNA序列,挖掘其SNPs位点,并成功将杂合SNP位点定位在遗传连锁群上。研究结果显示,通过序列比对在亲本间检测到3个SNPs,验证得到1个杂合的位点SNP735。将此位点成功转化为dCAPS标记,该标记在子代中的基因型分离比为1∶1,利用该群体已构建的茶树遗传图谱进行遗传定位,将dCAPS标记定位在连锁群LG03上,相邻标记为TM299和TM517。联合此标记及其相邻标记与游离氨基酸总含量和茶氨酸含量进行统计分析,表明具有显著的相关性。
The theanine synthetase(TS)gene is considered as the key functional gene for the synthesis of theanine intea plant.In this study,an F1segregating population and its parents were used to detect single nucleotidepolymorphism(SNP)in the TS gene for genetic mapping.According to the sequence alignment between the parents,three SNPs were found,and SNP735was identified to be heterozygous in YS.SNP735was subsequentlytransformed into dCAPS marker and used for genotyping in the F1population.The results showed that thesegregation ratio of alleles at this SNP locus was close to1:1.The dCAPS marker was mapped to LG03at a positionbetween TM299and TM517in the tea genetic map.Meanwhile,significant correlations between the dCAPS markerand the content of theanine and total amino acid were identified in this study.
作者
李小杰
马建强
姚明哲
陈亮
LI Xiaojie;MA Jianqiang;YAO Mingzhe;CHEN Liang(Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou 310008, China)
出处
《茶叶科学》
CAS
CSCD
北大核心
2017年第3期251-257,共7页
Journal of Tea Science
基金
国家茶叶产业技术体系项目(CARS-023)
国家自然科学基金(31170624
31500568)
