摘要
本实验以从江西婺源绿茶中提取的茶多糖(tea polysaccharides,TPS)为研究材料,研究不同剂量TPS对正常小鼠肠道健康及免疫调节功能的影响。实验小鼠随机分为正常组和低、中、高剂量TPS干预组。TPS干预21 d后,收集小鼠腹腔巨噬细胞,进行中性红吞噬实验检测腹腔巨噬细胞的吞噬能力,解剖获取小鼠脾脏、胸腺后称其质量并计算脾指数和胸腺指数。气相色谱法检测小鼠结肠内容物中短链脂肪酸(short chain fatty acids,SCFAs)的含量;靛酚蓝比色法测定结肠内容物中氨含量;p H计检测结肠内容物p H值;恒质量法检测结肠内容物含水量。结果显示:高、中、低剂量TPS干预组的小鼠免疫器官指数均显著增大,腹腔巨噬细胞吞噬能力呈剂量依赖性增强,结肠内容物的乙酸,丙酸和正丁酸的含量升高,而异丁酸、正戊酸、异戊酸的含量无明显变化;结肠内容物中氨含量下降,结肠内容物p H值下降,含水量升高。实验结果提示TPS具免疫调节功能及促进小鼠肠道健康的作用。
The objective of this work was to evaluate the effect of tea polysaccharides(TPS,from Wuyuan green tea)on intestinal health and immune regulation in mice.Healthy C57/BL6mice were randomly divided into four groups,and they were administrated by gavage with different concentrations of tea polysaccharides.After adaptive feeding for one week,the animals in these groups were given high,mediumand low doses of TPS and normal saline as blank control for21days,respectively.At the end of the experimental period,the mice were sacrificed,and spleen and thymus were harvested and weighted to calculate immune organ indexes.Peritoneal macrophages were used to examine phagocytosis activity by using neutral red phagocytosis assay.Short chain fatty acids(SCFAs)in colonic contents were analyzed by gas chromatography(GC)and ammonia by the indophenol blue colorimetric method.Meanwhile,the pH and water content of colonic contents were also measured.Results showed that immune organ indexes were increased significantly after TPS administration.The phagocytosis of peritoneal macrophages was improved in a dose-dependent manner.Some SCFAs(acetic acid,propionic acid and butyric acid)were increased while no difference in isobutyrate,pentanoic acid and delphinic acid was detected.A significant decline in the ammonia content and pH of colonic contents of mice was observed,while water content increased.Therefore,TPS can regulate immune function and promote intestinal health in mice.
作者
李海珊
刘丽乔
聂少平
LI Haishan;LIU Liqiao;NIE Shaoping(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第7期187-192,共6页
Food Science
基金
国家自然科学基金优秀青年科学基金项目(31422042)
关键词
茶多糖
免疫调节
肠道健康
tea polysaccharides
immune regulation
intestinal health
