摘要
目的探讨高危型HPV感染基因型在宫颈病变组织中的分布情况。方法采用核酸分子快速导流杂交基因芯片技术,对382例宫颈组织样本进行HPV的基因分型检测。结果在382例宫颈组织样本中高危型HPV的阳性率为51.3%(196/382),19~30岁、31~40岁、41~50岁和>50岁年龄组高危型HPV的阳性率分别为41.1%、61.1%、56.3%和43.2%,各年龄组间差异无统计学意义(P>0.05)。将宫颈上皮内瘤样变(CIN)及以上病变作为病理学阳性诊断,在196例高危型HPV阳性者中,病理学阳性154例,阳性率为78.6%(154/196);在186例高危型HPV阴性者中,病理学阳性19例,阳性率为10.2%(19/186),2个组差异有统计学意义(P<0.05)。在196例高危型HPV阳性者中共检出13种亚型,检出率由高到低排在前5位的为16、58、18、52和33型,各型阳性率差异无统计学意义(P>0.05)。结论高危型HPV感染与宫颈病变的发生、发展密切相关,徐州地区高危型HPV感染的亚型主要为16、58、18、52和33型。
Objective To investigate the distribution of high-risk human papillomavirus (HPV) genotypes in cervical lesion samples. Methods A total of 382 cervical lesion samples were collected for HPV genotyping by molecular rapid diversion hybridization gene chip technology. Results The positive rate of high-risk HPV was 51.3%(196/382). The positive rates in patients with 19-30,31-40,41-50 and >50 years old were 41.1%,61.1%,56.3% and 43.2%,respectively,but there was no statistical significance for different age groups (P>0.05). Using cervical intraepithelial neoplasia (CIN) grade or worse as pathological positive diagnosis,there were 154 cases of pathological positive,and the positive rate was 78.6% (154/196) among 196 cases of high-risk HPV positive samples. There were 19 cases of pathological positive among 186 cases of high-risk HPV negative samples with positive rate of 10.2%(19/186). There was statistical significance between the 2 groups (P<0.05). A total of 13 subtypes were found from 196 cases of high-risk HPV positive samples. The top 5 subtypes of determination rates from high to low were 16,58,18,52 and 33,however,the positive rates with different subtypes had no statistical significance (P>0.05).Conclusions High-risk HPV infection is tightly related with the occurrence and development of cervical lesion,and the top 5 subtypes of high-risk HPV infection are 16,58,18,52 and 33 in Xuzhou.
作者
丁静
王泉
王庆国
DING Jing;WANG Quan;WANG Qingguo(Department of Clinical Laboratory,the First People's Hospital of Xuzhou, Xuzhou 221002,Jiangsu,China)
出处
《检验医学》
CAS
2017年第3期199-201,共3页
Laboratory Medicine
基金
徐州市医学科研资助项目(XWJ2011007)
