摘要
利用细菌复合菌系LDC对木质纤维素进行生物处理,研究其分解及产酶特性、细菌组成多样性,为秸秆类木质纤维素资源的生物利用提供依据。用范氏纤维素测定方法测定复合菌系LDC在降解芦苇的过程中木质纤维素含量变化,同时检测分解过程中的发酵液的p H及酶活力变化趋势;运用高通量技术对LDC的16Sr DNA基因的V3-V4区进行测序,分析其细菌组成多样性。LDC具有较强的木质素、半纤维素分解能力,而很少分解纤维素;LDC能够分泌木质素降解酶,漆酶、木质素过氧化物酶、锰过氧化物酶的最大酶活分别为136.7、1 206.5、3 933.3 U·L-1。LDC秸秆周围的液态培养物(S1)及LDC处理后的秸秆表面(S2)两个样品的细菌组成没有差异、菌属组成丰度存在很大差异。S1中相对量最高的优势菌属为索氏菌属(Thauera,22.32%);S2中相对量最高的优势菌属为:假单胞菌属(Pseudomonas,26.99%)。LDC不分解纤维素且具有较高效的木质素分解能力,对研究木质素的生物降解具有非常重要的意义。
In order to better use the compound bacteria strains LDC to treat lignocellulose resources,it was important to research its characteristics of decomposition,producing enzyme and the diversity of bacteria composition.The change of lignocellulose content during the process of reed degradation with LDC was detected by using the Fann cellulose determination method. The change tendency of pH and enzyme activity of fermentation liquid in the process of reed degradation was detected. The V3-V4 region of 16SrDNA gene of LDC was sequenced by using high throughput technology to analyze the diversity of bacteria composition.The LDChad strong ability of lignin and hemicellulose decomposition,but weak ability of cellulose decomposition. The LDC could secretelignin degradation enzymes and the maximal enzyme activity of laccase,lignin peroxidase and manganese peroxidase was 136.7,1 206.5 and 3 933.3 U·L-1 respectively. There was no difference in the bacteria composition of the two samples consisting liquidcultures(S1)and the surface of straw treated by LDC(S2). However,there was a big difference in the composition abundance ofbacteria genus both S1 and S2. The dominant bacterium of the highest relative amount was Thauera in the S1(Thauera,22.32%).The dominant bacterium of the highest relative amount was Pseudomonas in the S2(Pseudomonas,26.99%).The LDC had very important significance for the study of lignin biodegradation in cellulose decomposition with high efficiency.
作者
李莹
艾士奇
曹文悦
王海鹏
熊志强
王国兴
王伟东
Li Ying;Ai Shiqing;Cao Wenyue;Wang Haipeng;Xiong Zhiqiang;Wang Guoxing;Wang Weidong(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2017年第1期83-88,共6页
journal of heilongjiang bayi agricultural university
基金
国家科技支撑计划(2015BAD21B04
2012BAD12B05-3)
黑龙江省高校科技创新团队项目(2012TD006)
黑龙江农垦总局攻关项目(HNK125B-11-06A
HNK125B-11-11A)
关键词
木质素分解
复合菌系
木质素降解酶
多样性
lignin degradation
bacterial community
lignocellulolytic enzymes
diversity of bacterial compositio
