摘要
目的探讨血浆同型半胱氨酸（Hcy）水平及其代谢酶5,10-亚甲基四氢叶酸还原酶（MTHFR）基因多态性与老年原发性高血压合并冠心病的关系。方法选择2014年2月~2015年2月于玉林市第一人民医院心内科住院的老年原发性高血压患者共200例，其中男性115例，女性85例，平均年龄（67.52±3.53）岁。根据冠状动脉造影结果分为高血压组（100例）及冠状动脉粥样硬化性心脏病（冠心病）组（100例）。选取同时期的健康体检者100例为对照组。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法检测3组MTHFRC677T基因型，同时检测Hcy水平。结果与对照组比较，高血压组和冠心病组血压、三酰甘油、总胆固醇、低密度脂蛋白胆固醇、Hcy均升高，差异有统计学意义（P均＜0.05）。高血压组MTHFRC677位点CC、CT和TT基因型频率分别为27.0%、50.0%和23.0%，C和T等位基因频率分别为52.0%和48.0%。冠心病组CC、CT和TT基因型频率分别为12.0%、55.0%和33.0%，C和T等位基因频率分别为39.5%和60.5%。对照组CC、CT和TT基因型频率分别为40.0%、45.0%和15.0%，C和T等位基因频率分别为62.5%和37.5%。冠心病组TT型基因频率及T等位基因频率均高于高血压组和对照组，差异有统计学意义（P均＜0.05）。冠心病组患者TT型Hcy水平高于CC型和CT型，差异有统计学意义（P均＜0.05）。结论MTHFRC677T基因多态性中TT基因型可能是老年原发性高血压合并冠心病的一个重要遗传风险因子，T等位基因可能为该病的风险等位基因。
Objective To discuss the relationship between homocysteine (Hcy) or gene polymorphisms ofmethylenetetrahydrofolate reductase (MTHFR) and essential hypertension complicating coronary heart disease(CHD) in elderly patients. Methods The patients with essential hypertension (n=200, male 115, female 85 andaverage age=67.52±3.53) were chosen from the Department of Cardiology of First People’s Hospital of Yulin Cityfrom Feb. 2014 to Feb. 2015. The patients were divided, according to outcomes of coronary angiography (CAG),into hypertension group and CHD group (each n=100). At the same time, healthy controls (n=100) were chosenas control group. The genotypes of MTHFR C677T were detected by using polymerase chain reaction-restrictionfragment length polymorphism (PCR-RFLP) method and Hcy level was detected meanwhile in 3 groups. ResultsCompared with control group, blood pressure, triglyceride (TG), total cholesterol (TC), low-density lipoproteincholesterol(LDL-C) and Hcy increased in hypertension group and CHD group (all P<0.05). In hypertensiongroup, the frequency was 27.0% in CC genotype, 50.0% in CT genotype and 23.0% in TT genotype, and frequencywas 52.0% in C allele and 48.0% in T allele at MTHFR C677 locus. In CHD group, the frequency was 12.0% inCC genotype, 55.0% in CT genotype and 33.0% in TT genotype, and frequency was 39.5% in C allele and 60.5%in T allele. In control group, the frequency was 40.0% in CC genotype, 45.0% in CT genotype and 15.0% in TTgenotype, and frequency was 62.5% in C allele and 37.5% in T allele. The frequency of TT genotype and frequencyof T allele were higher in CHD group than those in hypertension group and control group (all P<0.05). Hcy levelwas higher in TT genotype than that in CC genotype and CT genotype in CHD group (all P<0.05). Conclusion TTgenotype of MTHFR C677T maybe an important genetic risk factor, and T allele maybe a risk allele for CHD inelderly patients with essential hypertension.
作者
赵艳梅
王东旭
许为炎
蔺晶晶
黄翠娟
王正东
梁萍
ZHAO Yan-mei;WANG Dong-xu;XU Wei-yan;LIN Jing-jing;HUANG Cui-juan;WANG Zheng-dong;LIANG Ping(Department of Cardiology, First People's Hospital of Yulin City, Yulin 537000, China)
出处
《中国循证心血管医学杂志》
2016年第10期1191-1194,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
玉林市科学研究与技术开发计划项目(1421036)
