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酶联免疫斑点法和ELISA法诊断结核病临床价值的探讨 被引量:5

Clinical Value of ELISPOT and ELISA Method in Diagnosis of Tuberculosis
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摘要 目的:探讨酶联免疫斑点法(ELISPOT)和ELISA法诊断结核病的临床价值。方法:86例疑似结核病患者,同时行ELISPOT和ELISA法的检验,对结果做配对卡方检验及Kappa一致性检验,比较两种方法的检验的结果,探讨临床诊断应用的价值。结果:配对卡方检验,P值为0.774,>0.05,不拒绝H0,二者无差异。Kappa一致性检验,Kappa值为0.579,0.4<Kappa值≤0.75表示一致性中等。结论:两种方法检验结果差异无显着性,具有一致性,结合实验操作性,方便性,ELISA法更适合应用于临床。 Objective: To investigate the clinical value of enzyme linked immunosorbent assay (ELISPOT) and ELISAmethod in the diagnosis of tuberculosis. Methods: 86 cases of suspected tuberculosis patients. At the same time, the ELISPOTand ELISA test, the paired chi square test and kappa consistency test, to compare the two methods of test results of the value ofclinical application. Results:Paired chi square test, P value was 0.774,>0.05, does not reject H0, no difference between the two.Kappa consistency test, Kappa = 0.579, Kappa>0.4 or lower than 0.75 medium consistency.Conclusion:There were no significantdifferences between the two methods. The results showed that the ELISA method was more suitable for clinical application.
作者 石海萍 周晓芹 Shi Hai-ping;Zhou Xiao-qin(Department of Internal Seven, Shanxi Tuberculosis Prevention and Control Hospital, Xi'an 710100,China;Department of Infectious Disease, Xunyi County Hospital of Shanxi Province, Xunyi 711300, China)
出处 《中国医药导刊》 2016年第7期651-652,共2页 Chinese Journal of Medicinal Guide
关键词 结核病 酶联免疫斑点法 ELISA法 临床价值 Tuberculosis ELISPOT ELISA Clinical value
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