摘要
在华北宝力格油田水处理系统的三相分离器与反应池之间加入激活剂,采用16S r DNA高通量测序技术,研究三相分离器、反应池、过滤器和喂水泵4个水处理节点水样中微生物(细菌和古菌)群落结构及多样性,并分析不同水处理节点水样中驱油功能菌含量。研究结果表明,各水处理节点水样的细菌和古菌群落结构存在差异。三相分离器、反应池、过滤器和喂水泵处水样中细菌群落中的优势菌分别为弯曲菌科、红长命菌属、红长命菌属和固氮弧菌属、地衣芽孢杆菌属;三相分离器和喂水泵处水样中古菌群落中的优势菌均为甲烷食甲基菌属。喂水泵处细菌和古菌多样性较前一个水处理节点减小,说明微生物群落结构由复杂向简单发展。喂水泵处驱油功能菌含量最大,这些驱油功能菌注入地下后可发挥驱油作用。
In the present work,the structures and diversities of the microbial communities(bacteria and archaebacteria)inthe water samples collected from the three-phase separator,the reaction tank,the filter and waterflood pump of the watertreatment system in Baolige oilfield were studied through the high-throughput 16S rDNA sequencing technique when acti?vating agents were added between the three-phase separator and reaction tank. In addition,the distribution and content ofthe oil recovery microbes in the four water samples were also analyzed. Results showed that the community structures of thebacteria and archaebacteria varied with the site of the water sample collected. The dominant bacterial groups in threephaseseparator,reaction tank,filter and waterflood pump were Campylobacteraceae,Tepidicella,Tepidicella,Azoarcus andBacillus licheniformis,respectively. The dominant archaebacterial groups in the three-phase separator and the waterfloodpump were both Methanomethylovorans. The diversity of the bacteria and archaebacteria in the water sample collected fromthe waterflood pump was lower than that in the previous site of sample collection,which indicates that the microbial commu?nity structure became simple compared with the previous water sample collection site. The concentration of the oil recovery microbes in the waterflood pump was the largest,which can be beneficial to the oil recovery when they are injected into theoil reservoir.
作者
游靖
徐建昌
余吉良
孙霜青
李春玲
胡松青
You Jing;Xu Jianchang;Yu Jiliang;Sun Shuangqing;Li Chunling;Hu Songqing(School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai City,200237,China;Production Engineering Research Institute,PetroChina Huabei Oilfield Company,Renqiu City,Hebei Province,062552,China;College of Science,China University of Petroleum(East China),Qingdao City,Shandong Province,266580,China)
出处
《油气地质与采收率》
CAS
CSCD
北大核心
2016年第5期76-82,共7页
Petroleum Geology and Recovery Efficiency
基金
中国石油重大科技专项"华北油田上产稳产800万吨关键技术研究与应用"(2014E-3507)
关键词
微生物群落结构
多样性
16S
rDNA高通量测序
驱油功能菌
激活剂
microbial community structure
diversity
high-throughput 16s rDNA sequencing technique
oil recovery microbes
activating agent
