摘要
采用反相高效液相色谱法 ,对膳食补充剂中的维生素C、叶酸、泛酸的同时测定进行了方法学研究。色谱参考条件为SupelcosilC18柱 ;柱温 :4 0℃ ;检测波长 :2 10nm ;流动相 :0 .0 5mol/LKH2 PO4∶CH3 OH∶0 .1mol/LKOH =85 +8+7(pH6 .3) ;流速 :1.5ml/min。用外标法定量 ,线性范围为维生素C :0～ 1.0mg/ml;叶酸 :0～ 0 .1mg/ml;泛酸 :0～ 0 .2 5mg/ml。线性相关系数为 0 .999到 0 .9997,平均回收率为 90 .5 %～ 10 5 .6 % ,相对标准偏差为 1.6 %～ 8.0 %。方法简便 ,快速、准确 ,用于实际样品测定 ,结果令人较为满意。
A reversed phase high performance liquid chromatography for the simultaneous determination of vitamin C. folic acid, pantothenic acid in dietary supplements was developed. The optimized chromatographic conditions were: Supelcosil C 18 column, column temperature: 40℃, detection wavelength: 210nm, mobile phase: 0.05mol/L KH 2 PO 4 :CH 3 OH:0.1mol/L KOH=85+8+7(pH6.3), flow rate: 1.5ml/min.The quantitative analysis of the three vitamins was achieved with external standard method . The linear range was 0～1.0mg/ml for ascorbic acid , 0～0.1mg/ml for folic acid and 0～0.25mg/ml for pantothenic acid. The correlation coefficients ranged from 0.999 to 0.9997. The average recovery of the method was 90.5%～105.6%. The relative standard deviation was 1.6%～8.0%. The method was simple, rapid and accurate. It was applied for detecting actual samples with satisfactory result.
出处
《安徽预防医学杂志》
2002年第6期336-338,共3页
Anhui Journal of Preventive Medicine
关键词
反相高效液相色谱法
膳食补充剂
维生素C
reversed phase high performance liquid chromatography
vitamins
dietary supplements
