摘要
目的探讨获得性免疫缺陷综合征(acquired immunodef iciency syndrome,AIDS)合并慢性扁桃体炎患者外周血和扁桃体组织中Th1/Treg应答情况。方法采用RT-PCR检测了外周血和扁桃体T-bet/Foxp3的表达;同时采用免疫组织化学法检测T-bet/Foxp3以及CD4/C D 8细胞的数量;采用全血胞内细胞因子染色方法,分析CD4+IFN-γ+T/CD4+CD25+Foxp3+细胞表达水平。结果FACS检测A IDS伴慢性扁桃体炎患者CD4+IF N-γ+T细胞表达(20.65±7.14)%显著低于健康对照(39.13±13.30)%和慢性扁桃体炎患者(36.89±10.05)%;CD4+CD25+Fo x p 3+细胞表达(7.38±2.6 3)%则明显高于健康对照(4.31±2.14)%和慢性扁桃体炎患者(4.79±2.23)%;CD4细胞计数(104.5±60.96)显著低于健康对照(663.9±170.3)和慢性扁桃体炎患者(546.4±151.9);CD8细胞计数(580.5±130.7)显著高于健康对照(364.7±69.85)和慢性扁桃体炎患者(443.0±150.5);RT-PCR检测AIDS伴慢性扁桃体炎患者T-bet表达(4.831±2.61)显著低于健康对照(20.12±11.31)和慢性扁桃体炎患者(9.83±3.54);Foxp3表达(102.5±47.42)显著高于健康对照(57.28±25.19);免疫组化检测AIDS伴慢性扁桃体炎患者扁桃体组织中T-bet表达(80.20±2.20)显著高于慢性扁桃体炎患者(31.9±2.82);Foxp3表达和慢性扁桃体炎患者差异无统计学意义;CD4细胞(42.09±4.09)显著低于慢性扁桃体炎患者(80.93±3.42);CD8细胞(80.45±2.81)显著高于慢性扁桃体炎患者(37.57±3.11)。结论患者外周血和扁桃体组织中存在明显不同的Th1/Treg免疫反应,Th1/Treg失衡在AIDS伴慢性扁桃体炎患者致病机制中起着重要作用。
OBJECTIVE To investigate the Th1 and Treg response in peripheral blood and tonsil tissue from patients with AIDS/Tonsillitis. METHODS The expression of T-bet and Foxp3 were evaluated with RT-PCR in peripheral blood and tonsil from patients with AIDS/tonsillitis, chronic tonsillitis and healthy control. T-bet/Foxp3 and CD4/CD8 expression in tonsillitis tissues were detected using immunohistochemistry method. The frequency of CD4+IFN-γ+ and CD4+CD25+Foxp3+ T cells were analyzed with Flowcy tometry. RESULTS The frequency of CD4+IFN-γ+ T cells was significantly decreased in patient with AIDS/Tonsillitis, compared with chronic tonsillitis and healthy control. The frequency of CD4+CD25+Foxp3+ T cells was significantly increased in patient with AIDS/ tonsillitis. The absolute CD4 number in patient with AIDS/ tonsillitis was significantly lower than that in chronic tonsillitis or healthy control and the absolute CD8 number was significantly higher in patient with AIDS/Tonsillitis than that in patients with chronic tonsillitis or healthy control by flow cytometry analysis. The similar results were observed in tonsil tissue using immunohistochemistry method. Patients with AIDS/ tonsillitis had a significantly decreased expression of T-bet but a significantly increased Foxp3 expression compared with healthy control. However, the immunohistochemistry results showed that the expression of T-bet in tonsil tissue was significantly increased in AIDS/Tonsillitis. There was no difference in Foxp3 expression between AIDS/Tonsillitis and chronic tonsillitis. CONCLUSION The results suggested that Th1 and Treg response from patient with AIDS/Tonsillitis are different between peripheral blood and tonsil tissue, and indicated that Th1 and Treg may play an important role in the pathogenesis of patients with AIDS/Tonsillitis.
出处
《中国耳鼻咽喉头颈外科》
CSCD
2015年第6期311-314,共4页
Chinese Archives of Otolaryngology-Head and Neck Surgery
