摘要
目的 :研究烫伤后心肌组织胞浆型磷脂酶 A2 (c PL A2 ) m RNA变化 ,探讨其与心肌细胞膜损伤的关系及意义。方法 :采用大鼠 4 0 %体表面积 度烫伤模型 ,伤后 0 (正常组 )、1、3、6、12、2 4小时不同时间点测定组织及血浆中肌酸激酶同工酶 (CK MB)活性及组织膜磷脂含量 ;采用逆转录聚合酶链反应 (RT PCR)技术检测c PL A2 m RNA水平的变化。结果 :与正常组比较 ,烫伤后大鼠血浆 CK MB活性明显升高的同时 ,伴组织 CKMB活性下降 ,以 6和 12小时最为明显 (P均 <0 .0 1) ;组织膜磷脂含量伤后 3小时显著降低 (P<0 .0 1) ,6小时降至最低 ;c PL A2 伤后 1小时表达明显增强 ,2 4小时仍维持高表达水平。膜磷脂含量变化与 c PL A2 m RNA、CK MB变化存在显著相关 (P<0 .0 5和 P<0 .0 1)。结论 :上调表达的 c PL A2 加剧细胞膜磷脂降解可能是烫伤后心肌损害的重要原因之一。
Objective:To investigate the expression changes in cytosolic phospholipase A 2 (cPLA 2)in myocardium and explore its possible role in cell membrane injury.Methods:After 40% total body surface area (TBSA)Ⅲ degree burn injury,the expression of cPLA 2 was detected by reverse transcription polymerase chain reaction(RTPCR) at different time points.Changes in total phospholipid content in myocardial membrane of rats were evaluated by changes in phosphorus contents in membrane,and plasma creatine kinase isoenzyme (CKMB), as well as tissue CKMB activity were estimated.Results:A marked increase in CKMB in plasma was found,accompanied by a lowering of its content in myocardium.mRNA level of cPLA 2 in tissue was increased significantly after 1 hour postburn.Meanwhile,cell membrane phospholipid contents were decreased 3 hours after the injury.It showed a remarkable relationship with changes in both cPLA 2 expression and CKMB activity.Conclusions:The upregulation of cPLA 2 expression may be responsible for myocardial lesion through aggravation of membrane phopholipid degradation.
出处
《中国危重病急救医学》
CAS
CSCD
2002年第10期588-590,共3页
Chinese Critical Care Medicine
基金
国家重点基础研究发展规划项目 (No.19990 5 42 0 2 )
国家杰出青年科学基金资助项目 (No.3 0 12 5 0 40 )
全军医药卫生科研基金资助项目 (No.0 1L0 66)
高等学校骨干教师资助计划项目
