摘要
目的了解依那普利拉对严重烫伤大鼠早期心肌力学的影响。方法将84只SD大鼠背部造成30％TBSA的Ⅲ度烫伤后，随机分为烫伤组，伤后按Parkland公式腹腔注射等渗盐水；小剂量治疗组、中剂量治疗组、大剂量治疗组，伤后即刻分别腹腔注射1、2、4mg／kg依那普利拉。烫伤组、小剂量治疗组伤后1、3、6、12、24h，中剂量治疗组、大剂量治疗组伤后6、12h左心室置管检测大鼠左心室收缩压（LVSP）、左心室舒张末期压（LVEDP）、左心室压力最大上升／下降速率（±dp／dtmax），并处死大鼠取心肌组织检测血管紧张素Ⅱ（AⅡ）含量。另取6只大鼠作为假伤组，模拟烫伤后检测以上指标。结果伤后3～24h，烫伤组及各剂量治疗组大鼠LVSP、LVEDP、±dp／dt max值普遍低于假伤组（P〈0．05或P〈0．01）；而各剂量治疗组LVSP、LVEDP、±dp／dt max值普遍高于烫伤组（P〈0．05或P〈0．01）；伤后6、12h，大剂量治疗组±dp／dtmax明显低于小、中剂量治疗组。伤后1h，烫伤组心肌组织AⅡ含量[（53．0±2．6）pg／200mg]明显高于假伤组[（14．8±0．7）pg／200mg，P〈0．01]，6h达高峰，以后逐渐下降，伤后24h仍明显高于假伤组（P〈0．01）；伤后3～24h，小剂量治疗组AⅡ含量均明显高于假伤组（P〈0．05或P〈0．01），但均低于烫伤组。伤后6h烫伤组AⅡ含量为（145．2±14．5）pg／200mg，高于小、中、大剂量治疗组[（65．1±0．9）、（53．6±1．1）、（34．2±0．9）pg／200mg，P〈0．01]。结论严重烫伤后早期心肌组织损害明显，心功能即明显下降，依那普利拉注射液可以改善心肌力学指标、保护心功能，以小剂量效果最为明显。
Objective To investigate the therapeutic effects of Enalaprilat on the myocardial kinetics in rats at early stage of severe scald . Methods Eighty-four SD rats were inflicted with 30 % TBSA fullthickness scald, and randomly divided into scald （S,with intraperitoneal injection of isotonic saline according to Parkland formula ,n=30） ,L（n=30） ,M（n=12） and H （n=12） groups. The rats in L ,M,H groups were intraperitoneally injected with 1,2,4 mg/kg Enalaprilat. Other 6 healthy rats were enrolled into study as control（ C group）. The myocardial kinetic parameters including left ventricular systolic pressure （LVSP） , left ventricular end diastolic pressure（LVEDP） , ± dp/dt max and the levels of AⅡ in myocardium were observed at 1,3,6,12 and 24 post scald hour（PBH） in L and S groups,and at 6,12 PBH in M and H groups. The above indices in C group were also examined. Results The levels of LVSP, LVEDP, ± dp/dt max in C group were higher than those in other groups during 3～24 PBH （ P 〈0.05 or P 〈0.01 ） ,while those in L,M,H groups were obviously higher than those in S group （ P 〈0.05 orP 〈0.01 ）. The level of ±dp/ dt max in H group at 6,12 PBH were obviously lower than those in L and M groups. The level of AⅡ in S group at 1 PBH was （ 53.0 ± 2.6） pg/200 mg , which was significantly higher than thatin C group [ （ 14.8 ± 0.7） pg/200 mg, P 〈 0.05 or P 〈 0.01 ] ;it peaked at 6 PBH and lowered afterwards, and they were significantly higher than that in C group at 24 PBH （ P 〈 0.01 ）. The levels of AⅡ in L group during 3～24 PBH were obviously higher than those in C group （ P 〈0.01 ） ,which were also lower than those in S group. The level of AⅡ in S group was significantly higher than in L,M, H groups at 6 PBH [ （145.2 ± 14.5） pg/200 mg. vs. （65.1±0.9） pg/200 mg,（53.6±1.1） pg/200mg,（34.2±0.9） pg/200 mg,respectively, P 〈0.01 ]. Conclusion Myocardium can be obviously damaged at early stage after severe scald, cardiac function
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2008年第3期183-186,共4页
Chinese Journal of Burns
基金
国家重点基础研究发展规划（2005CB522601）
国家自然科学基金重点项目（30430680）
全军医学科学技术研究“十一五”计划专项课题（062033）
关键词
烧伤
依那普利拉
心肌力学
Burns
Enalaprilat
Myocardial kinetics
