摘要
采用PVP Pd IrO2 Nafion修饰电极对成纤维细胞中NO的释放情况进行了研究 .结果表明 ,在正常状态下 ,采用NO前体L 精氨酸和乙酰胆碱对成纤维细胞进行刺激后没有NO的释放 ;当用脂多糖进行诱导后 ,则释放出高浓度的NO ,加入L 精氨酸和乙酰胆碱都促进了NO的合成 ,而L NNA的加入则逆转了L 精氨酸和乙酰胆碱的作用 .
PVP/Pd/IrO2/Nafion microsensor was fabricated to measure nitric oxide production in fibrocytes directly. In the experiments, fibrocytes did not release nitric oxide after stimulated with L-arginine and acetylcholine under normal condition. After induced with hpopolysaccharide, NO was produced in high concentration and NO levels were increased after stimulated with L-arginine and acetylcholine. On the other hand, L-N-w-nitro-arginine, an NO synthase inhibitor, decreased the action of L-arginine and acetylcholine.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2002年第8期1459-1464,共6页
Acta Chimica Sinica
基金
国家自然科学基金 (No .2 9975 0 0 9)
上海市自然科学基金 (No .0 0ZA14 0 11)资助项目
