摘要
目的对不同碘摄入水平地区妇女的血液氧化还原指标进行调查,初步探讨碘摄入量对血液氧化还原能力的影响。方法筛选建立适碘地区和高碘地区调查现场。收集当地水源水,以及调查对象的空腹晨尿及静脉血,采用砷-铈催化分光光度法测定水碘和尿碘,采用化学发光法测定血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平,采用酶联免疫吸附测定法测定超氧化物歧化酶(SOD,superoxide dismutase)和丙二醛(MDA,Malondialdehyde)。结果高碘地区的水碘、尿碘显著高于适碘地区(P<0.05),高碘地区水碘、尿碘分别为294.1μg/L、564.5 (326.0~896.3)μg/L,适碘地区为72.3μg/L、142.3 (93.6~242.0)μg/L。血清FT3水平分别为(4.77±0.50)pmol/L、(4.95±1.63)pmol/L,高碘地区较低(P<0.05);FT4水平分别为(15.26±1.77)pmol/L、(14.57±2.97)pmol/L,高碘地区较高(P<0.01);TSH水平在高碘地区和适碘地区之间无统计学差异(P>0.05),分别为2.36 (1.564~3.432) mIU/mL和2.29 (1.64~3.21) mIU/mL。SOD水平分别为2.70 (1.84~7.13) pg/ml、8.12 (2.49~56.03) pg/ml,高碘地区较低(P<0.01);MDA水平分别为0.28 (0.20~0.60) pg/ml、0.44 (0.32~2.97) pg/ml,高碘地区较低(P<0.01)。经相关性分析发现SOD和MDA均与尿碘水平呈负相关(r=-0.195,P<0.05;r=-0.402,P<0.01),而与年龄、FT3、FT4和TSH之间没有相关性。结论高碘地区妇女的血清氧化还原指标SOD和MDA水平均低于适碘地区,长期碘摄入过量可能会影响血液的氧化还原平衡体系。
Objective To investigate the blood REDOX index of women in areas of different iodine intake levels,then explore the effect of iodine excess intake on REDOX capacity of blood.Method Survey spots of iodine sufficient area and iodine excess area were chosen.Local source water,limosis morning-urinary and venous blood were collected.The iodine contents in water and urine were detected by arsenic and cerium catalysis spectrophotometric method.Chemilu-minescent immunoassay was used to perform free triiodothyronine(FT3),free thyroxine(FT4),and thyroid-stimulating hormone(TSH)in serum.Superoxide dismutase(SOD)and Malondialdehyde(MDA)were measured by Enzyme-linked immunosorbent assay(ELISA).Result The water iodine in the iodine excess intake area were higher than those in the iodine sufficient area(P<0.05).For urine iodine,the iodine excess area was higher(P<0.05),with the value of 564.5(326.0~896.3)μg/L vs 142.3(93.6~242.0)μg/L.The level of FT3 in the iodine excess area was lower(P<0.05),with the value of(4.77±0.50)pmol/L vs(4.95±1.63)pmol/L.The level of FT4 in the iodine exces area was higher(P<0.05),with the value of(15.26±1.77)pmol/L vs(14.57±2.97)pmol/L.No significant differences were found in the TSH level and the incidence of thyroid functional abnormality between the two areas.The level of SOD in the iodine excess area was lower(P<0.01),with the value of 2.69(1.84~7.13)pg/ml vs 8.124(2.49~56.03)pg/ml.And the level of MDA in the iodine excess area was also lower(P<0.01),with the value of 0.28(0.20~0.60)pg/ml vs 0.44(0.32~2.97)pg/ml.SOD and MDA were found negatively correlated with urine iodine by correlation analysis(r=-0.195,P<0.05;r=-0.402,P<0.01),while the others were not related.Conclusion The serum SOD and MDA of women in iodine excess area is higher than those in iodine sufficient area.Long-term intake of excess iodine may affect the REDOX balance system in blood.
作者
曹晓晓
李秀维
张莹
王海燕
王建强
马巍
徐菁
CAO Xiao-xiao;LI Xiu-wei;ZHANG Ying;WANG Hai-yan;WANG Jian-qiang;MA Wei;XU Jing(Key Laboratory of Trace Element,National Health Commission of the People's Republic of China,Beijing 100050,China)
出处
《中国地方病防治》
CAS
2019年第3期241-244,共4页
Chinese Journal of Control of Endemic Diseases
基金
2018年国家财政项目“碘营养状况评估与甲状腺疾病调查”
