摘要
利用 RT- PCR技术从顿河红豆草含浓缩单宁的愈伤组织总 RNA中分离克隆了凝集素 c DNA.该 c NDA包含完整的编码区和 3’端非编码区 ,所编码的氨基酸序列与其它豆科植物凝集素具有较高的同源性。
A cDNA fragment was isolated and cloned from the total RNA of calli of Onobrychis tanaitica containing condensed tannin using RT-PCR technique.The cDNA is 919 bp in length and includes an open reading frame encoding peptide of 251 amino acid residues and 163 bp of 3' uncoding region.The deduced amino acid sequence shows high homology to those of other legume leactins.
出处
《草地学报》
CAS
CSCD
2002年第3期221-226,共6页
Acta Agrestia Sinica
基金
国家自然科学基金项目 ( C0 2 0 30 10 2 )
