摘要
对顿河红豆草５ｄ龄无菌苗下胚轴愈伤组织诱导及其植株再生条件的研究结果表明，愈伤组织培养基为ＭＳ基础培养基，附加０．２ｍｇ／ｌ２，４－Ｄ、１ｍｇ／ｌＢＡＰ和２ｍｇ／ｌＮＡＡ，ｐＨ５．８；分化培养基为无激素ＭＳ培养基；生根培养基为１／２ＭＳ或１／２ＬＳ＋０．０５ｍｇ／ｌＮＡＡ＋０．８％琼脂培养基。其愈伤组织绝大多数生长良好，出愈率达９６．８％。体细胞胚分化率为２５．３％，具有体细胞胚分化力的基因型（幼苗）占３５％，生根率不到１０％。
In China, Onobrychis tanaitica Spreng., only distributes in Tianshan Mountains, Altai mountain ect. in Xinjiang. As it is a diploid species and its leaf contains high condensed tannins, it is a bloat-free legume and a good breeding material for protoplast fusion with alfalfa, identification of the proteins related to condensed tannin biosynthesis and condensed-tannin gene(s) clone. This paper deals with the 5-day seedling and plant regenertion of O. tanaitica. The results showed that the callus culture medium was MS medium supplemented with 0.2mg/l 2,4-D, 1mg/l BAP and 2mg/l NAA (pH 5.8, 0.8% agar). Differentiation medium was hormone free MS medium and the rooting medium was 1/2 MS or 1/2 LS supplemented with 0.05mg/l NAA and 0.8% agar. The most of calli grow well and the callus induction frequency was 96.8%. The somatic embryo induction rate (somatic embryo induced calli/total calli) and the somatic embryo induced genotype rate (somatic embryo induced seedlings/total cultured seedlings) sere 25.3% and 35%, respectively. However, the rooting rate was less than 10%. The rooting conditions was the problem to be studied in the future.
出处
《中国草地》
CSCD
1998年第5期18-20,25,共4页
Grassland of China
关键词
顿河红豆草
下胚轴培养
体细胞胚
植株再生
Onobrychis tanaitica Spreng.,\ Tissue culture,\ Somatic embryo,\ Plant regeneration
