摘要
目的 :探讨不同浓度IGF 1对兔成骨细胞的成骨影响。方法 :采用组织块细胞培养技术获得兔成骨细胞 ,第 2代成骨细胞分别在含 0 .1ng/ml、1ng/ml、10ng/ml、2 0ng/mlIGF 1的DMEM中培养 ,2 4、36h后行MTT法检测细胞增殖情况 ,72、10 8h收集培养上清进行骨钙素放射免疫法 (RI)测定。结果 :IGF 1与成骨细胞培养 2 4、36h ,经MTT法检测 ,不同浓度IGF 1与对照组比较 ,有显著性差异 (P <0 .0 0 1) ;IGF 1浓度为 0 .1、1、10ng/ml,各组之间相比存在显著性差异 (P <0 .0 5 ) ;IGF 1与成骨细胞培养 72、10 8h ,经RI检测 ,不同浓度IGF 1对成骨细胞合成骨钙素与对照组比较无显著性差异 (P >0 .0 5 )。结论 :IGF 1对成骨细胞有明显促进增殖作用 ;在 0 .1～ 10ng/ml范围 ,存在浓度依赖性 ;未发现IGF 1对成骨细胞合成骨钙素有影响。
Objective: To investigate the effects of IGF-1 on bone formation of rabbit osteoblast in vitro.Methods:Osteoblasts were obtained from rabbit costal bone by explant culture technique.The second subculture was exposed to IGF-1 at variant concentrations of 0.1,1,10 and 20ng/ml for 24 and 36 hours,and the effects of IGF-1 on osteoblast proliferation was observed by MTT assay.The contents of BGP were measured by the radioimmunoassay in the culture supernatant harvested after the second subculture were exposed to IGF-1 for 72 and 108 hours.Results:Osteoblast exposed to IGF-1 for 24 and 36 hours at variant concentrations of 0.1,1,10,20ng/ml proliferated significantly compared with the control (P<0.001),and significant differences were found between every two groups when osteoblast were exposed to IGF-1 at range from 0.1 to 10ng/ml(P<0.05).Levels of osteocalcin in culture supernatant of osteoblast exposed to IGF-1 at variant concentrations for 72 and 108 hours was not statistically significant compared with control group (P>0.05).Conclusions:IGF-1 could promote the proliferation of osteoblast,and there were dose-dependent relationship at a limited range from 0.1 to 10ng/ml.IGF-1 had no effect on osteocalcin synthesis of osteoblast.
出处
《中国矫形外科杂志》
CAS
CSCD
2002年第11期1104-1105,共2页
Orthopedic Journal of China
