摘要
目的:研究胰岛素样生长因子1(IGF-1)对大鼠精原细胞MAPK途径分子Erk1和2磷酸化的影响作用.方法:分离并培养大鼠精原细胞,采用不同浓度的IGF-1刺激培养的精原细胞.采用Western Blot方法检测Erk1/2分子的磷酸化.结果:成功获得体外培养的精原细胞,IGF-1刺激后精原细胞Erk1/2分子的磷酸化水平显著增强.IGF-1浓度与Erk1/2磷酸化水平呈正相关关系.结论:IGF-1增强Erk1/2分子的磷酸化水平可能是IGF-1促进精原细胞增殖和提高大鼠生精能力的重要分子机制.
AIM： To investigate the influence of cytokine IGF-1 on the phosphorylation situation of MAPK pathway molecular Erkl and Erk2 in spermatogenous cell. METHODS： Separate and transiently culture the rat spermatogenous cell, the phosphorylation of Erkl/2 was detected with Western Blot in spermatogenous cell treated with serials concentrations of IGF-1. RESULTS： The spermatogenous cells were obtained successfully and transciently cultured for more than 12 h. Cytokine IGF-1 could enhance the phosphorylation level of MAPK pathway molecular Erkl/2 in various degree. The higher the concentration of IGF-1 is, the more intensive the phosphorylation of Erkl/2 is. CONCLUSION： Cytokine IGF-1 can promote the proliferation of spermatogenous cells by elevating the phosphorylation level of Erkl/2. These suggest the IGF-1 is an important factor to support the spermatogenic capability.
出处
《第四军医大学学报》
北大核心
2009年第20期2143-2145,共3页
Journal of the Fourth Military Medical University
