摘要
大鼠气管内注入低剂量脂多糖 (LPS)制成慢性支气管炎模型。利用光镜和透射电镜方法并结合图像分析技术观察各组大鼠气道病理形态学变化。结果 :假手术组大鼠气道形态无明显异常改变。模型组大鼠出现气道内分泌物明显增多 ,气道腺体增厚 ,上皮不完整 ,气管粘膜层、粘膜下层大量炎细胞浸润 ,支气管外周淋巴滤泡形成 ;图像分析显示 :模型组大鼠气管壁厚度、气管腺体厚度均较假手术组显著增加 (P <0 0 1 ) ;肺脏超微结构显示 :肺泡腔大量炎性细胞渗出 ,肺泡间隔增厚。艾麻口服液组气道病理损伤较模型组明显减轻 ;图像分析显示 :艾麻口服液组气管壁厚度明显变薄(P <0 0 5 )。橘红止咳液组气道病理损伤较模型组亦有所减轻 ,但作用不如艾麻口服液显著。结论
Male Wistar rats were randomly divided into the sham operation (SO) group, model group, Aima Oral Solution (AOS) group and Juhongzhike Oral Solution (JOS) group, and each group consisted of 9 animals. The rat model of chronic bronchitis was induced by the intratracheal injection of a small dose of lipopolysaccharide. Optical and transmission electron microscopic methods and image analysis were used to observe airway pathomorphological changes in the rats in the above 4 groups. The results showed that there were no obvious abnormal morphological changes in the airway of the animals in the SO group; but in the animals in the model group, the secretion in the airway was obviously increased, the glands of the airway were thickened, the epithelium of the airway was damaged, tracheal mucous and submucous layers were infiltrated by a large quantity of inflammatory cells, and the peribronchial lymph follicles were formed. Image analysis revealed that the thickness of the tracheal wall and tracheal glands was significantly increased in the animals in the model group as compared with that in the SO group ( P <0 01). The observation of the lung ultrastructure revealed that the alveolar cavities were infiltrated by a large quantity of inflammatory cells and the alveolar interstitium was thickened in the animals in the model group; but the airway pathologic injuries were obviously relieved in the AOS group as compared with those in the model group. Image analysis showed that the thickness of the tracheal wall in the animals in the AOS group was significantly decreased ( P <0 05). Although the airway pathologic injuries were also relieved by JOS as compared with those in the model group, the efficacy of JOS was not as good as that of AOS.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2002年第5期26-29,共4页
Journal of Beijing University of Traditional Chinese Medicine
关键词
艾麻口服液
慢性支气管炎
大鼠
气道
病理损伤
Lipopolysaccharide
Chronic Bronchitis
Aima Oral Solution
Pathology
Image Analysis
Rat
