摘要
目的:研究外源NO对胰岛细胞的损伤机制和功能的影响。方法:采用DNA电泳技术以及单细胞电泳检测NO对胰岛细胞的凋亡和DNA的损伤作用;通过测定胰岛素含量检测胰岛细胞在NO作用下的合成和分泌功能。结果:外源NO能导致胰岛细胞DNA的断裂形成彗星细胞,DNA电泳显示典型梯状条带,大剂量NO形成死亡条带;同时NO能抑制胰岛细胞合成和分泌胰岛素。结论:外源NO可导致胰岛细胞凋亡、DNA断裂损伤并抑制其合成分泌胰岛素的功能。
<Abstrcat>Objective:To study the mechanism of the destruction and changes of function of islet cells caused by exogenous nitric oxide(NO).Methods:The damage of DNA and apoptosis in islet cells were assessed in vitro with single cell gel electrophoresis and gel electrophoresis of DNA. Insulin synthesis and secretion of normal rats pancreas under high level ofglucose caused by NO were detected.Results:The exogenous NO caused breakage of DNA strand .The islet cells showed remarkable apoptotic ladder with low dose of NO and necrosis ladder with high dose.The insulin synthesis and secretion of islet cells under high level of glucose were inhibited by NO in vitro.Conclusion:The exogenous NO can directly induce islet cells apoptosis,DNA breaking and inhibit their function.
出处
《西北国防医学杂志》
CAS
2002年第5期361-363,共3页
Medical Journal of National Defending Forces in Northwest China
基金
.NULL.
